Effect of calcium hydroxide on proinflammatory cytokines and neuropeptides
ABSTRACT Calcium hydroxide, a widely used intracanal medicament, is known to exert an antimicrobial effect and to degrade bacterial-derived lipopolysaccharides. However, little is known about the effect of Ca(OH)(2) on endogenous inflammatory mediators such as interleukin-1 alpha (IL-1 alpha), tumor necrosis factor-alpha (TNF-alpha), and calcitonin gene-related peptide (CGRP). This is an important gap in knowledge because these inflammatory mediators play an important role in mediating the pathogenesis of periradicular periodontitis. We tested the hypothesis that Ca(OH)(2) denatures IL-1 alpha, TNF-alpha, and CGRP. Human IL-1 alpha (0.125 ng/mL), TNF-alpha (0.2 ng/mL), and CGRP (0.25 ng/mL) were incubated with Ca(OH)(2) (0.035 mg/mL) for 1-7 days. At the end of the incubation period, the pH of the samples was neutralized, and the concentrations of the mediators were measured by immunoassays. Data were analyzed with one-way analysis of variance and Bonferroni multiple comparison tests. The results indicate that Ca(OH)(2) denatures IL-1 alpha, TNF-alpha, and CGRP by 50%-100% during the testing periods (P < .001). We concluded that denaturation of these proinflammatory mediators is a potential mechanism by which Ca(OH)(2) contributes to the resolution of periradicular periodontitis.
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- "This could be attributed to the continuous release of hydroxyl and calcium ions with the continuous ionic dissociation of the tested calcium hydroxide formulations and its antiinflammatory action. These findings were in agreement with the results of a previous study (Khan et al., 2008) and in disagreement with another study (Filho et al., 2002) which concluded that all calcium formulations causing an inflammatory response. "
ABSTRACT: The aim of the present work is to study the effect of different formulations of Ca (OH) 2 on healing of induced periapical lesions in dog. A total of 96 teeth with intentionally induced periapical lesions were classified according to the observation period into three groups; I, II and III (2 dogs each). Each group was subdivided into four subgroups (8 teeth each) namely; A, B, C and D which were dressed with Ca(OH) 2 with saline, Ca(OH) 2 with chlrohexidine, Ca(OH) 2 with iodoform and control respectively. Histopathological findings showed that the apical and periapical repair were better in subgroup A than in other subgroups in all groups. Total inflammatory cell count was significantly different between the four subgroups in group I. In both groups II and III, there was no significant difference between subgroups B and C. In conclusion, the use of saline as a vehicle for Ca (OH) 2 has a favorable action on periapical tissue healing in endodontically treated dogs. ©2012 PVJ. All rights reserved To Cite This Article: El-Ashry S, A. Abu-Seida, H. Al-Boghdady, K. El-Batouty and M Abdel-Fattah, 2013. The effect of different formulations of calcium hydroxide on healing of intentionally induced periapical lesions in dogs. Pak Vet J, 33(1): 48-52.Pakistan Veterinary Journal 01/2012; 8318:2074-7764. · 1.39 Impact Factor
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ABSTRACT: The aim of this study was to evaluate morphologic microstructure and surface hardness of white mineral trioxide aggregate (WMTA) after exposure to a range of alkaline environments during hydration. WMTA was mixed and packed into 60 glass tubes. Four groups, each containing 15 tubes, were exposed to pH values of 7.4, 8.4, 9.4, and 10.4, respectively, for 3 days. In 12 tubes in each group, Vickers surface hardness was measured after exposure to alkaline environments. Data were subjected to one-way analysis of variance and a post hoc Tukey test. Three specimens in each group were prepared to be evaluated under a scanning electron microscope using scattered electron (SE) and backscattered electron (BSE) detectors. The mean surface hardness values +/- standard deviation after exposure to pH values of 7.4, 8.4, 9.4, and 10.4 were 58.28 +/- 8.21, 68.84 +/- 7.19, 67.32 +/- 7.22, and 59.22 +/- 9.14, respectively. The difference between these values was statistically significant (p = 0.000). There were statistically significant differences between pH values of 8.4 and 9.4 and pH values of 7.4 and 10.4 (p > 0.05). The SE detector revealed needle-shaped crystals at pH values of 7.4 and 8.4 and an amorphous microstructure at pH values of 9.4 and 10.4 on WMTA surface. The BSE detector showed more unhydrated structure and pores at pH values of 7.4 and 10.4 compared with pH values of 8.4 and 9.4. Surface hardness can be influenced by different alkaline pH values. The BSE detector can reveal more microstructure details of WMTA in conjunction with the SE detector. More porosity and unhydrated structure are observed in WMTA exposed to pH values of 7.4 and 10.4.Journal of endodontics 06/2009; 35(5):706-10. DOI:10.1016/j.joen.2009.01.017 · 2.79 Impact Factor
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ABSTRACT: The aim of this study was to evaluate the effect of a range of alkaline pH values on the push-out strength of white mineral trioxide aggregate (WMTA). The standardized lumens of root slices prepared from extracted single-rooted human teeth were filled with white ProRoot MTA. The specimens were then randomly divided into 4 groups (n = 20) and wrapped in pieces of gauze soaked in synthetic tissue fluid (STF) (pH, 7.4) and STF buffered in potassium hydroxide at pH values of 8.4, 9.4, or 10.4. The samples were incubated for 3 days at 37 °C. The push-out bond strengths were then measured by using a universal testing machine. Failure modes after the push-out test were examined under a light microscope at ×40 magnification. The data were analyzed by using one-way analysis of variance and Tukey post hoc tests. The greatest (9.46 ± 0.63 MPa) and lowest (5.68 ± 0.83 MPa) mean push-out bond strengths were observed after exposure to pH values of 8.4 and 10.4, respectively. There were significant differences between the groups (P = .001). The bond failure was adhesive for all experimental groups. Push-out bond strength of WMTA could be influenced by different alkaline pH values.Journal of endodontics 11/2010; 36(11):1856-9. DOI:10.1016/j.joen.2010.08.022 · 2.79 Impact Factor