Sex determination of jojoba (Simmondsia chinensis cv. Arizona) by random amplified polymorphic DNA (RAPD) molecular markers

AFRICAN JOURNAL OF BIOTECHNOLOGY (Impact Factor: 0.57). 01/2011;

ABSTRACT Jojoba (Simmondsia chinensis (Link) Schneider) is a dioecious shrub that produces fruits in female
plants. Its seeds stores liquid wax which is used in cosmetic, pharmaceutical and plastic industries.
This species is generally propagated by seed. The sex of seedlings is not distinguishable by cytological
and seed cultivation methods. This investigation was carried out to study the sex-specific random
amplified polymorphic DNA (RAPD) markers in thirteen 4-year-old jojoba plants from two provinces of
Iran and DNAs of those populations were extracted by CTAB method. Out of the 20 tested primers, two
primers, namely F1 and F10, produced 460 and 680 bp fragments, respectively and were importantly
recognized to distinguish between female and male plants, accordingly. Also, the results of the ratio
difference test showed that, more efficient sex determination of jojoba seedlings is done using both F1
and F10 primers due to gene cooperation between them. The preliminary results of this study for sex
determination would help the recognition of potential fruit-bearing seedlings for having high yield per
hectare in horticultural systems. Furthermore, the findings would help saving time and economic
resources in jojoba breeding programs.

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