The development of chagasic megacolon requires severe denervation and the reduction in interstitial cells of Cajal number might be a contributing factor

Discipline of Special Pathology, Universidade Federal do Triângulo Mineiro (UFTM), Av. Getúlio Guaritá, 130, 38025-440, Uberaba, Minas Gerais, Brazil, .
Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin (Impact Factor: 2.65). 12/2012; 462(1). DOI: 10.1007/s00428-012-1349-1
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    • "process that damages intramural plexus with a reduction in ganglion nerve fibers of both the myenteric and submucosal plexuses. This damage also affects cholinergic transmission, which becomes severely impaired in megacolon (Adad et al. 2013). The T. cruzi inflammatory process produces interleukins that affect muscle contractility (Aubé et al. 1996; Hu et al. 2007) by stimulating the release of cholinesterase in response to acetylcholine (Ach) production. "
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    ABSTRACT: This study evaluated the inflammatory process in the colons of mice infected with Trypanosoma cruzi QM2 strain, through the analysis of muscle reactivity and the measurement of butyrylcholinesterase (BuChE) in plasma. "Swiss" mice were infected with T. cruzi QM2 strain and after 15 (G15), 30 (G30), 60 (G60), 90 (G90), and 210 (G210) days, each group had blood collected for the measurement of butyrylcholinesterase plasma concentrations ([BuChE]), a measure which functioned as an indicator of plasmatic Ach levels. All groups, except G15, had a segment of proximal colon removed to assess muscle reactivity to acetylcholine (Ach) and noradrenaline (NA) stimulation. After reactivity tests, the tissues were then fixed and stained with hematoxylin and eosin (HE) for histological evaluation of inflammatory response. The QM2 strain did induce inflammatory process in mice colon, and demonstrated differences in muscular contraction between the G60 and G210 groups, with p < 0.05. Plasma [BuChE] increased during the acute phase of infection (p < 0.05) with subsequent heterogeneous decline in the late chronic phase. These results show that the QM2 strain has tropism to the colon of mice and causes damage characteristic of megacolon; also, Ach has an enigmatic importance in the anti-inflammatory reflex over the course of T. cruzi infection.
    Parasitology Research 04/2014; 113(6). DOI:10.1007/s00436-014-3861-5 · 2.10 Impact Factor

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