Phytofabrication of silver nanoparticles by using aquatic plant Hydrilla verticilata

Nusantara Bioscience 07/2012; 4:2087-3948.

ABSTRACT Sable N, Gaikwad S, Bonde S, Gade A, Rai M. 2012. Phytofabrication of silver nanoparticles by using aquatic plant Hydrilla verticilata. Nusantara Bioscience 4: 45-49. In the context of current drive to developed new green technology in nanomaterials, synthesis of nanoparticles is of considerable importance. There has been considerable work done in the field of nanoscience and Nanotechnology during the last decade due to the introduction of various protocols for the synthesis of nanoparticles by using plants and microorganisms. Here we firstly report the extracellular phytosynthesis of silver nanoparticles (Ag-NPs) using aquatic plants Hydrilla verticilata. The characterization of the phytosynthesized Ag-NPs was done with the help of UV-Vis spectroscopy, FTIR, Nanoparticle Tracking Analysis (NTA), Zeta potential and SEM. The SEM micrograph revealed the synthesis of polydispersed spherical nanoparticles, with the average size of 65.55 nm. The phytofabricated Ag-NPs can be used in the field of medicine and agriculture, due to their antimicrobial potential. Abstrak. Sable N, Gaikwad S, Bonde S, Gade A, Rai M. 2012. Fitofabrikasi nanopartikel perak menggunakan tumbuhan akuatik Hydrilla verticilata. Nusantara Bioscience 4: 45-49. Dalam konteks mendorong pengembangan teknologi hijau yang baru pada nanomaterial, sintesis nanopartikel sangat penting. Selama dekade terakhir terjadi perkembangan yang cukup pesat dalam bidang nanosains dan nanoteknologi karena diperkenalkannya berbagai protokol untuk mensintesis nanopartikel menggunakan tumbuhan dan mikroorganisme. Dalam penelitian ini, dilaporkan fitosintesis ekstraseluler nanopartikel perak (Ag-NP) menggunakan tumbuhan akuatik Hydrilla verticilata untuk pertamakalinya. Karakterisasi Ag-NP yang difitosintesis dilakukan dengan bantuan spektroskopi UV-Vis, FTIR, Analisis Pelacakan Nanopartikel (NTA), potensial Zeta dan SEM. Mikrograf SEM menunjukkan hasil sintesis nanopartikel berbentuk bulat yang tersebar, dengan ukuran rata-rata 65,55 nm. Fitofabrikasi Ag-NP dapat dimanfaatkan dalam bidang kedokteran dan pertanian, karena memiliki potensi antimikroba.

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    ABSTRACT: 2012): Murraya koenigii-mediated synthesis of silver nanoparticles and its activity against three human pathogenic bacteria, Nanoscience Methods, 1:1, 25-36 This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae, and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand, or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material. Synthesis of silver nanoparticles (Ag NPs) by the leaf extract of Murraya koenigii (Indian curry leaf tree) is reported in this study. The colour of the leaf extract prepared by grinding turned from green to brown after treatment with AgNO 3 (1 mM). The UV–visible spectroscopic analysis showed the absorbance peak at about 420 nm, which indicates the synthesis of Ag NPs. Further characterisation by Fourier transform infrared spectroscopy showed the presence of proteins as capping agents, which increase the stability of Ag NPs in the colloids. Scanning electron microscopy demonstrated the presence of spherical Ag NPs in the range of 40–80 nm. The bactericidal activity of the standard antibiotics was significantly increased in the presence of Ag NPs against pathogenic bacteria, viz., Escherichia coli-JM-103 (ATCC 39403), Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (MTCC 424). Ag NPs in combination with gentamicin showed the maximum activity against E. coli (increase in fold area À4.06), followed by P. aeruginosa (1.11) and S. aureus (0.09), while tetracycline showed maximum activity against S. aureus (2.16) followed by P. aeruginosa (0.24) and E. coli (0.21). The Ag NPs thus obtained demonstrated remarkable antibacterial activity against three human pathogenic bacteria when used in combination with commercially available antibiotics.
    Nanoscience methods. 04/2012; 1:25-36.


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