Polyphenols excreted in urine as biomarkers of total polyphenol intake

Nutrition & Food Science Department, XaRTA, Instituto de Investigaciónen Nutrición y Seguridad Alimentaria, Pharmacy School, University of Barcelona, Barcelona, Spain.
Bioanalysis (Impact Factor: 3). 11/2012; 4(22):2705-13. DOI: 10.4155/bio.12.249
Source: PubMed


Nutritional biomarkers have several advantages in acquiring data for epidemiological and clinical studies over traditional dietary assessment tools, such as food frequency questionnaires. While food frequency questionnaires constitute a subjective methodology, biomarkers can provide a less biased and more accurate measure of specific nutritional intake. A precise estimation of polyphenol consumption requires blood or urine sample biomarkers, although their association is usually highly complex.

This article reviews recent research on urinary polyphenols as potential biomarkers of polyphenol intake, focusing on clinical and epidemiological studies. We also report a potentially useful methodology to assess total polyphenols in urine samples, which allows a rapid, simultaneous determination of total phenols in a large number of samples.

This methodology can be applied in studies evaluating the utility of urinary polyphenols as markers of polyphenol intake, bioavailability and accumulation in the body.

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    • "The validity of the FFQ to assess total polyphenol intake was studied using total polyphenol excretion in spot urine samples in a clinical trial (r Z 0.48, P < 0.01) and in a cross-sectional study (r Z 0.26, P Z 0.04) [16]. This range is likely an underestimate of the true validity because spot urine polyphenol excretion likely best represents intake from the previous 3e12 h whereas the FFQ captures average intake over the previous year [17]. Daily food and nutrients intake was estimated from the FFQ by multiplying the frequency of consumption by the average portion size. "
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    Nutrition Journal 09/2015; 14(1). DOI:10.1186/s12937-015-0083-3 · 2.60 Impact Factor