Article

Reduced number and impaired function of circulating endothelial progenitor cells in patients with abdominal aortic aneurysm

Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan
International journal of cardiology (Impact Factor: 6.18). 11/2012; 168(2). DOI: 10.1016/j.ijcard.2012.11.002
Source: PubMed

ABSTRACT AIM: Circulating endothelial progenitor cells (EPCs) are associated with coronary artery disease (CAD) and predict its outcome. Although the pathophysiology of abdominal aortic aneurysm (AAA) is different, it shares some risk factors with CAD. Therefore, the correlation between EPCs and AAA was investigated. METHODS AND RESULTS: Seventy-eight subjects (age 77.2±7.8years) with suspected AAA were prospectively enrolled. Cut-off values (men, 3.5-5.5cm; women, 3-5cm) were used to define normal aorta, small AAA, and large AAA on thoraco-abdominal computer tomography. Endothelial function was measured by flow-mediated vasodilation (FMD). Flow cytometry and colony-forming units (CFUs) were used to evaluate circulating EPC numbers. Circulating EPCs were defined as mononuclear cells with low CD45 staining and double-positive staining for KDR, CD34, or CD133. Late out-growth EPCs were cultured from six patients with large AAAs and six age- and sex-matched controls to evaluate proliferation, adhesion, migration, tube formation, and senescence. FMD was significantly lower with large (5.26%±3.11%) and small AAAs (6.31%±3.66%) than in controls (8.88%±4.83%, P=0.008). Both CFUs (normal 38.39±12.99, small AAA 21.22±7.14, large AAA 6.98±1.97; P=0.026) and circulating EPCs (CD34(+)/KDR(+) and CD133(+)/KDR(+)) were significantly fewer in AAA patients than in controls. On multivariate analysis, CFUs and circulating EPCs (CD34(+)/KDR(+)) were independently, inversely correlated to AAA diameter. Proliferation, adhesion, migration, tube formation, and senescence of late EPCs were significantly impaired in AAA patients. CONCLUSION: The number and function of EPCs were impaired in AAA patients, suggesting their potential role in AAA.

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    • "Endothelial dysfunction has been associated with decreased circulating endothelial progenitor cells (1,2) and increased circulating endothelial microparticles (3,4). Both conditions are influenced by classic risk factors (4-6) and inflammatory status (7,8). "
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    • "Previous vascular repairing and regeneration studies using EPCs usually focused on coronary and peripheral vasculature injury. They showed a precise treatment efficacy and many prospective applications[9], [10], [11], [12], [13], [14], [15]. Recently, growing studies have confirmed that EPCs, as an important factor in the process of systemic vascular protection and restoration, play a critical role in endothelial maintenance, the vascular repairing process, and postnatal vasculogenesis[16], [17], [18]. "
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