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The inhibitory efficiency of the essential oil from lemon grass and citronella grass on the growth of Bovine mastitis pathogens: Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli

Khon kaen Agriculture Journal 05/2012;

ABSTRACT The objective of the study was to investigate the inhibitory effect of the essential oils extracted from lemon grass and citronella grass on the growth of three bovine mastitis pathogens: Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli. The essential oil contents extracted from lemon grass and citronella grass by hydro-distillation method were 1.76 and 1.28% (w/v), respectively. The inhibitory effect of those selected plant essential oils on three mastitis pathogenic bacteria was studied by 10-fold broth dilution technique at the concentration ranging from 0.3906 to 200 ug/ml. Results showed that, among the bacteria tested against essential oil of lemon grass, S. aureus and St. agalactiae were found to be highly sensitive followed by E.coli. Minimal inhibitory concentrations (MIC) of lemon grass oil against S. aureus, St. agalactiae and E. coli were 3.125, 3.125 and 12.5 ug/ml and the minimum bactericidal concentrations (MBC) of lemon grass oil against those bacteria were 6.25, 25.0 and 25.0 ug/ml, respectively. The essential oil extracted from citronella grass had the ability to inhibit the growth of S. aureus, St. agalactiae and E. coli at MIC of 1.5625, 3.125 and 6.25 ug/ml and MBC of 6.25, 6.25 and 25 ug/ml, respectively. These results indicate that the essential oils extracted from lemon grass and citronella grass have the potential antibacterial activity against three mastitis pathogens. Therefore, the further investigation of application of those plant essential oils for mastitis control and treatment at farm level should be emphasized.

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