Article

High-Throughput RNA In Situ Hybridization in Mouse Retina.

The Salomon H. Snyder Department of Neuroscience, John Hopkins University School of Medicine, Baltimore, MD, USA, .
Methods in molecular biology (Clifton, N.J.) (Impact Factor: 1.29). 01/2013; 935:215-226. DOI: 10.1007/978-1-62703-080-9_15
Source: PubMed

ABSTRACT The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. The use of large-scale nonradioactive RNA in situ hybridization makes this possible, and greatly increases the general usefulness of this data. Here, we describe protocols for parallel analysis of up to 50 different gene-specific probes in mouse retinal sections.

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