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    • "The parasites collected were preserved in alcohol 70%. Some parasite specimens were mounted unstained in Hoyer's medium in order to study the sclerotized structures, while others were stained with Gomori's trichrome to observe internal organs (EIRAS et al., 2006). "
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    ABSTRACT: With the aim of creating an inventory of the metazoan gill parasites of Salminus hilarii in the Taquari River, state of São Paulo, Brazil, five species of monogeneans (Anacanthorus contortus, A. bicuspidatus, Annulotrematoides parisellei, Jainus iocensis and Tereancistrum arcuatus) are reported the first time for this host. A total of 28 fish were sampled quarterly between April 2011 and January 2012, with 10 hosts in a lentic ecosystem and 18 in a lotic ecosystem. Quantitative ecological descriptors (prevalence, intensity of infestation and abundance) were calculated for the purpose to comparing the two ecosystems sampled (lentic and lotic ecosystems). However, no quantitative difference between the lentic and lotic ecosystems was observed. The present study has made available a checklist for species of the genus Anacanthorus and their hosts and geographical distribution in the Neotropical region up to the present time.
    Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria 12/2013; 22(4):579-87. DOI:10.1590/S1984-29612013000400020 · 0.96 Impact Factor
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    • "The nematodes collected were killed in a heated 0.9 % saline solution and preserved in alcohol glycerin (Eiras et al. 2000). Then, these were clarified with lactophenol for identification , counted, measured, and photographed using IM50 software (Leica). "
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    ABSTRACT: The objective of the present study was to discover the hematological indices of Hoplias malabaricus infected by larvae of Contracaecum sp. A total of 105 fish were collected from two lakes located in the municipal district of Pirassununga, São Paulo, Brazil, of which 95.2 % were infested by L3 larvae of Contracaecum sp., with an average intensity of 348.7 ± 231.55 helminths/fish. A total of 76 fish were analyzed to establish hematological parameters. Following analysis, it was found that there was a significant difference between hematological parameters Hct, erythrocytes (Er), thrombocytopenia (p = 0.01), and Hb (p = 0.02) in lakes A and B, while there was no significant difference for leukocyte (p = 0.68), mean corpuscular volume, mean corpuscular hemoglobin (p = 0.06), and mean corpuscular hemoglobin concentration (p = 0.47). The intensity of infection correlated negatively with Er (r s = -0.42; p < 0.01) and positively for (r s = 0.48; p < 0.01). The high intensity of infection by Contracaecum sp. in H. malabaricus did not affect the health of the fish despite causing alterations in hematological variables.
    Parasitology Research 05/2013; 112(8). DOI:10.1007/s00436-013-3446-8 · 2.33 Impact Factor
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    • "They were then measured (total and standard lengths) and weighed. Parasites were collected and processed as described by Eiras et al. (2006), and were identified at the lower taxonomic level in accordance with Thatcher (2006) and Travassos et al. (1928). The parasitological indexes were calculated as recommended by Bush et al. (1997). "
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    ABSTRACT: The objective of the present study was to evaluate the parasite fauna of four species of ornamental fish collected in the Chumucuí River, municipality of Bragança, Pará, Brazil. From June 2006 to December 2007. Fishes (n=307) belonging to four species were collected, including 23 specimens of Moenkhausia sanctaefilomenae (redeye tetra), 37 Carnegiella strigata (marbled hatchetfish), 7 Chilodus punctatus (spotted headstander), and 240 Astyanax bimaculatus (twospot astyanax). The parasites found belonged to three taxa: monogeneans in the gills, nematodes (larvae of Capillaria sp. and Contracaecum sp.) in the digestive tract and liver and acanthocephalans (Quadrigyrus torquatus, Q. brasiliensis and Q. nickoli) in the stomach and intestine. Astyanax bimaculatus presented higher prevalence of acanthocephalans in the wet season, and lower prevalence of nematodes in the dry season. The possible importance of these parasites in the exportation of ornamental fish is discussed.
    Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria 03/2013; 22(1):34-38. DOI:10.1590/S1984-29612013005000015 · 0.96 Impact Factor
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