The Tomato cis-Prenyltransferase Gene Family.

Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan, 48109, USA.
The Plant Journal (Impact Factor: 5.97). 11/2012; 73(4). DOI: 10.1111/tpj.12063
Source: PubMed


cis-Prenyltransferases (CPTs) are predicted to be involved in the synthesis of long-chain polyisoprenoids, all with >5 isoprene (C5) units. Recently, we identified a short-chain CPT, neryl diphosphate synthase (NDPS1), in tomato (Solanum lycopersicum). Here, we searched the tomato genome and identified and characterized its entire CPT gene family, which is composed of seven members (SlCPT1-7, with NDPS1 designated as SlCPT1). Six SlCPT genes encode proteins with N-terminal targeting sequences, which, when fused to the green fluorescent protein (GFP), mediated GFP transport to the plastids of Arabidopsis protoplasts. The SlCPT3-GFP fusion protein was localized to the cytosol. Enzymatic characterization of recombinant SlCPT proteins demonstrated that SlCPT6 produces Z,Z-FPP and SlCPT2 catalyzes the formation of nerylneryl diphosphate, while SlCPT4, SlCPT5, and SlCPT7 synthesize longer chain products (C25-C55). While no in vitro activity could be demonstrated for SlCPT3, its expression in the Saccharomyces cerevisiae dolichol biosynthesis mutant (rer2) complemented the temperature sensitive growth defect. Transcripts of SlCPT2, SlCPT4, SlCPT5, and SlCPT7 are present at low levels in multiple tissues, SlCPT6 is exclusively expressed in red fruit and roots, and SlCPT1, SlCPT3 and SlCPT7 are highly expressed in trichomes. RNA interference-mediated suppression of NDPS1 led to a large decrease in β-phellandrene (which is made from neryl diphosphate), with greater reductions achieved with the general 35S promoter compared to the trichome-specific MKS1 promoter. Phylogenetic analysis revealed CPT gene families in both eudicots and monocots and that all the short-chain CPTs from tomato (SlCPT1, SlCPT2, and SlCPT6) are closely linked to terpene synthase gene clusters. © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.

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Available from: Geng Yu, Oct 04, 2015
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    • "In higher plants, such as Arabidopsis thaliana (Kera et al. 2012; Surmacz and Swiezewska 2011) and Solanum lycopercicum (Akhtar et al. 2013), cis-prenyltransferases are encoded by a multigene family, and their transcripts are induced in response to various abiotic stimuli. These reports suggest that some cis-prenyltransferases and their products functions in different tissues and/or participates in different cellular processes in response to various environmental stimuli. "
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    • "In contrast, plants usually express several CPTs. Thus, seven genes encoding CPT producing almost exclusively short-chain polyisoprenoids have been identified in tomato (SlCPT1–7) [16]. Interestingly, only one CPT (LAA66) has been described in Lilium logiflorum [17] which in fact does not preclude the existence of a family of CPT encoding genes in this plant. "
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