Characterising vancomycin's immunotoxic profile using Swiss and CFW mice as an experimental model.

Immunotoxicology Research Group, Pharmacy Department, Science Faculty, Universidad Nacional de Colombia, Bogotá, Colombia.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Impact Factor: 2.11). 10/2008; 63(6):436-41. DOI: 10.1016/j.biopha.2008.08.021
Source: PubMed

ABSTRACT Immunotoxicology can lead to determining the adverse effects of different compounds on the immune system. Sometimes, many drugs (especially antibiotics) induce immune alterations, mainly auto-immunity. This study was aimed at determining vancomycin's immunotoxic effect by comparing the original molecule to two of the most used copies. Thirty-two mice from two murine strains (Swiss and CFW) were treated with three antibiotic formulations for studying its effect on splenic lymphoid and peripheral blood cell populations by using haemograms, flow cytometry and blastogenesis assays. The results indicated that vancomycin produces neutropenia and lymphocytosis in peripheral populations and that it induces a selective immunomodulatory effect on splenocyte sub-populations, depending on formulation and the strain so treated.

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    • "es cepas de ratones para el estu - dio de patrones de susceptibilidad o resistencia , la caracterización de la respuesta inmune frente al parásito y el estudio de diferentes alternativas terapéuticas y de efectos tóxicos posteriores al empleo de un tratamiento ( Cáceres & Tapia , 1993 ; Goto , 1995 ; Requena et ál . , 2000 ; Salay et ál . , 2007 ; Salguero et ál . , 2009 ; Swihart et ál . , 1995 ) . El ratón y el hámster , considerado hoy en día como el biomodelo de predilección debido a que presenta evoluciones clínicas similares a los humanos , se han usado en el estudio de nuevas alternativas terapéuticas y vacunales ( Cal - vopina et ál . , 2006 ; Croft et ál . , 2006 ; Gifawesen et ál . , 1989 ; He"
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    ABSTRACT: Animal biomodels have been used since ancient times in the study of physiological and pathological processes. This knowledge has been the baseline for many human studies and has contributed to the improvement in life quality of many people. In the last decades, important advances have been reached in the microbiological and genetic definition of these biomodels, in the facilities for their maintenance and also, in a parallel way, in the improvement of their welfare based in the three R’s principles (Replacement, Reduction, and Refinement). In the infectious disease research and within these, in the zoonotic diseases research, that are still the main cause for mortality and disability in a lot of countries, the animal biomodels have been fundamental in understanding physiopathological processes, in the development of new diagnostic tests, and also in their prevention and control with the development and testing of new vaccines, drugs and treatment protocols. In this paper, historical and technical aspects of this biomodels are reviewed and their essential role in the research of some infectious diseases of Veterinary Public Health importance is discussed.
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