Investigation of EROD, CYP1A immunopositive proteins and SOD in haemocytes of Chamelea gallina and their role in response to B[a]P.
ABSTRACT CYP1A sub-family represents the main form of cytochrome P450 involved in benzo[a]pyrene (B[a]P) detoxification, but there are no clear evidences about its presence in invertebrates. 7-Ethoxy resorufin O-deethylase (EROD) activity is strictly related to CYP1A presence, at the same time P450-dependent oxidative metabolism leads to reactive oxygen species (ROS) production, thought to be an important mechanism of pollutant-mediated toxicity in aquatic organisms. Superoxide dismutases (SODs), EROD and CYP1A activities and/or expressions were detected in haemocytes of pooled clams (Chamelea gallina) and cell-free haemolymph after 24 h, 7 and 12 days of exposure to 0.5 mg/L of B[a]P. After 24 h, B[a]P content was maximum in whole tissues. A 61 kDa band was recognized in haemocytes and cell-free haemolymph by polyclonal anti-fish CYP1A, while 53.5 and 63.8 kDa CYP1A immunopositive proteins were discriminate without differences of expression. Differently, EROD, MnSOD activity/expression and ECSOD expression decreased in haemocytes and haemolymph. C. gallina immune system presents an interesting response dose/time exposure of B[a]P and the 7 days condition highlights the major effects of xenobiotic action. The identification of basal EROD levels supports the possible presence of the CYP1A, never identified in C. gallina and more specifically never isolated in immune cells, as confirmed by CYP1A-immunopositive proteins identification.
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ABSTRACT: This paper presents a new formulation for the voltage distributions on radial transmission lines and a new decoupling technique to determine the optimal reactive termination of ordinary and radial transmission lines minimizing standing wavesElectrical Performance of Electronic Packaging, 1996., IEEE 5th Topical Meeting; 11/1996
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ABSTRACT: Mequindox (MEQ) is a synthetic quinoxaline 1,4-dioxides (QdNOs) derivative which can effectively improve growth and feed efficiency in animals. This study was to investigate the dose-dependent long-term toxicity in the adrenal of male rats exposed to 180 days of MEQ feed. Our data demonstrated that high doses of MEQ in the diet for 180 days led to adrenal damage and steroid hormone decrease, combined with sodium decrease and potassium increase in rat plasma. Significant changes of GSH and SOD in plasma were observed in the high doses (110, 275 mg/kg) groups. At the same doses, MEQ treatment down-regulated the mRNA levels of CYP11A1, CYP11B1 and CYP11B2 which located in mitochondria, but up-regulated mRNA levels of CYP21 and 3beta-HSD which located in endoplasmic reticulum. In conclusion, we reported the dose-dependent long-term toxicity of MEQ on adrenal gland in male rats, which raise awareness of its toxic effects to animals and consumers, and its mechanism may involve in oxidative stress and steroid hormone biosynthesis pathway.Toxicology Letters 10/2009; 191(2-3):167-73. DOI:10.1016/j.toxlet.2009.08.021 · 3.36 Impact Factor
Article: Bivalve immunity.[Show abstract] [Hide abstract]
ABSTRACT: Bivalves are comprised of animals unclosed in two shell valves, such as mussels oysters, scallops and clams. There are about 7,500 bivalve species and some ofthem are of commercial importance. Recently, interest in bivalve immunity has increased due to the importance in worldwide aquaculture and their role in aquatic environmental science and their position in phylogenetic research. This chapter provides a short review of bivalve immunity, including cellular and humoral immunity and the key components and the interactions involved in humoral immunity.Advances in Experimental Medicine and Biology 01/2010; 708:44-65. DOI:10.1007/978-1-4419-8059-5_3 · 2.01 Impact Factor