Article

Refinement of macaque synteny arrangement with respect to the official rheMac2 macaque sequence assembly.

Department of Genetics and Microbiology, University of Bari, Via Amendola 165/A, 70126, Bari, Italy.
Chromosome Research (impact factor: 3.09). 11/2008; 16(7):977-85. DOI:10.1007/s10577-008-1255-1 pp.977-85
Source: PubMed

ABSTRACT We have compared the synteny block organization of the official macaque genome sequence assembly (Jan. 2006; rheMac2) with an independent assembly that used a molecular cytogenetic approach. The mapping of four synteny segments, ranging in size from 4 Mb to 24 Mb, was found to be inconsistent between the two datasets. We specifically investigated these discrepancies by appropriate co-hybridization FISH experiments with validated reference probes located outside the area under study. We found that in the macaque rheMac2 release three synteny segments were wrongly mapped and one segment was incorrectly oriented.

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Keywords

appropriate co-hybridization FISH experiments
 
inconsistent
 
independent assembly
 
official macaque genome sequence assembly
 
synteny block organization
 
synteny segments
 
two datasets
 
validated reference probes