Article

Role of Ebola virus VP30 in transcription reinitiation.

INSERM, U758, Filovirus Laboratory, 21 Av. Tony Garnier, 69365 Lyon, Cedex 07, France.
Journal of Virology (Impact Factor: 4.65). 11/2008; 82(24):12569-73. DOI: 10.1128/JVI.01395-08
Source: PubMed

ABSTRACT VP30 is a phosphoprotein essential for the initiation of Ebola virus transcription. In this work, we have studied the effect of mutations in VP30 phosphorylation sites on the ebolavirus replication cycle by using a reverse genetics system. We demonstrate that VP30 is involved in reinitiation of gene transcription and that this activity is affected by mutations at the phosphorylation sites.

Full-text

Available from: Reynard Olivier, Aug 29, 2014
0 Followers
 · 
203 Views
  • Source
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The genus Ebolavirus comprises with clinically significant viral pathogens, namely Bundibugyo ebolavirus, Reston ebolavirus, Sudan ebolavirus, Tai Forest ebolavirus and Zaire ebolavirus causing deadly haemorrhagic fever in human with mortality rate approaching 90% and thus posses a potential global health risk. Ebola infection can be characterized by immune suppression results in disintegration of vascular, coagulation and immune system leading to multi organ failure and shock. The recent Ebola epidemic in Africa is spiraling out of control and no approved therapies/vaccines are available yet for its treatment. The genomes of Ebolavirus are approximately 18.8-19.0 kb long; encode both structural and non structural proteins. The structural proteins play key role in attachment, entry, stability, gene expression and pathogenesis of virus within the host. Therefore, in this in silico approach the complete genome sequences of five different species of Ebolavirus were used to characterize and investigate the putative promoter motifs. The promoter sequences were identified in all the species along with their name, sequence, weight and location within gene by PROSCAN Version 1.7. A grand total of 107 promoters were detected within different Ebolavirus genome; out of which 31, 29, 33, 10 and 4 promoter sequences were found in Bundibugyo ebolavirus, Reston ebolavirus, Sudan ebolavirus, Tai Forest ebolavirus and Zaire ebolavirus respectively. The genome size and G+C (%) of different Ebolavirus were almost equal. This current study may helps in identifying and analyzing various promoter motifs in Ebolavirus, thus understanding their roles in the regulation of gene expression, cell specificity and development. It may also be helpful in designing effective expression vector as well as live attenuated vaccine, hence inventing progressive target specific delivery system and fruitful gene therapy against different Ebolavirus species.
    Journal of Global Biosciences 04/2015; 4(3):1747-1757.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The genus Ebolavirus comprises with clinically significant viral pathogens, namely Bundibugyo ebolavirus, Reston ebolavirus, Sudan ebolavirus, Tai Forest ebolavirus and Zaire ebolavirus causing deadly haemorrhagic fever in human with mortality rate approaching 90% and thus posses a potential global health risk. Ebola infection can be characterized by immune suppression results in disintegration of vascular, coagulation and immune system leading to multi organ failure and shock. The recent Ebola epidemic in Africa is spiraling out of control and no approved therapies/vaccines are available yet for its treatment. The genomes of Ebolavirus are approximately 18.8-19.0 kb long; encode both structural and non structural proteins. The structural proteins play key role in attachment, entry, stability, gene expression and pathogenesis of virus within the host. Therefore, in this in silico approach the complete genome sequences of five different species of Ebolavirus were used to characterize and investigate the putative promoter motifs. The promoter sequences were identified in all the species along with their name, sequence, weight and location within gene by PROSCAN Version 1.7. A grand total of 107 promoters were detected within different Ebolavirus genome; out of which 31, 29, 33, 10 and 4 promoter sequences were found in Bundibugyo ebolavirus, Reston ebolavirus, Sudan ebolavirus, Tai Forest ebolavirus and Zaire ebolavirus respectively. The genome size and G+C (%) of different Ebolavirus were almost equal. This current study may helps in identifying and analyzing various promoter motifs in Ebolavirus, thus understanding their roles in the regulation of gene expression, cell specificity and development. It may also be helpful in designing effective expression vector as well as live attenuated vaccine, hence inventing progressive target specific delivery system and fruitful gene therapy against different Ebolavirus species.
    Journal of Global Biosciences 03/2015; 4(3):1747-1757.