Isolation and Purification of Lipids by Centrifugal Partition Chromatography

Department of Chemistry, Villanova University, 노리스타운, Pennsylvania, United States
Journal of liquid chromatography 11/1990; 13(18):3603-3614. DOI: 10.1080/01483919008049558


Methodologies for the separation of various lipid classes including free fatty acids (FA) and fatty acid ethyl esters (FAEE), monoglycerides (MG), diglycerides (DG), triglycerides (TG), phosphoglycerides (PG), and glycosphingolipids (GSL) by Centrifugal Partition Chromatography are presented. The fatty acid ethyl esters, Hexadecanoate, Octadecanoate, cis-9-Octadecenoate; and cis,cis-9,12-Octadecadienoate, and all-cis-9,12,15-Octadecatrienoate, were separated at 800 rpm; flow rate of 2.0 ml/min; using n-hexane/acetonitrile (1:1, v/v) as the solvent system and normal ascending and reversed descending elution modes respectively. The fatty acid ethyl esters, all-cis-5,8,11,14,17-Icosapentaenoate and all-cis-4,7,10,13,16,19-Docosahexaenoate, were separated at 600 rpm; flow rate of 3.3 ml/min; using n-hexane/methanol/water (1/0.9/0.1, v/v/v) as the solvent system and normal ascending mode. FA and MG; and DG and TG were separated at 600 rpm; 10

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