Substrate elasticity controls cell proliferation, surface marker expression and motile phenotype in amniotic fluid-derived stem cells

Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, NC 27157, USA.
Journal of the mechanical behavior of biomedical materials 10/2012; 17. DOI: 10.1016/j.jmbbm.2012.10.001
Source: PubMed


The physical cues presented to stem cells by the substrate on or in which they exist have been shown to play a crucial role in regulation of their behavior. Until recently, most research has focused on the effects of substrate elasticity on differentiation capability rather than maintenance of long-term proliferation and plasticity. The main goal of the present study is to study the interaction of amniotic fluid-derived stem (AFS) cells with growth substrata of different elasticity, which may extend their application potentials. Here, we investigate the effects of elastic modulus (E'), on AFS cell proliferation, morphology, cell surface marker expression, and autocrine stimulation of cell migration. AFS cells cultured on substrates of different E' exhibited significant changes in proliferation and morphology. Immunohistochemistry revealed increased expression of cell surface markers associated with mesenchymal stem cells (MSCs) (CD44, CD90, CD105, and N-cadherin) in cells cultured on softer substrates. Additionally, AFS cells cultured on softer substrates induced autocrine stimulation of migration. Therefore, tailoring the elastic modulus of biomaterials to specific stiffness values is an effective method to control stem cell properties, which may modulate the effectiveness of their therapeutic applications.

1 Follower
12 Reads
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to obtain nanomechanical properties of living cells focusing on human amniotic fluid stem (hAFS) cell using nanoindentation techniques. We modified the conventional method of atomic force microscopy (AFM) in aqueous environment for cell imaging and indentation to avoid inherent difficulties. Moreover, we determined the elastic modulus of murine osteoblast (OB6) cells and hAFS cells at the nucleus and cytoskeleton using force-displacement curves and Hertz theory. Since OB6 cell line has been widely used, it was selected to validate and compare the obtained results with the previous research studies. As a result, we were able to capture high resolution images through utilization of the tapping mode without adding protein or using fixation methods. The maximum depth of indentation was kept below 15% of the cell thickness to minimize the effect of substrate hardness. Nanostructural details on the surface of cells were visualized by AFM and fluorescence microscopy. The cytoskeletal fibers presented remarkable increase in elastic modulus as compared with the nucleus. Furthermore, our results showed that the elastic modulus of hAFS cell edge (31.6kPa) was lower than that of OB6 cell edge (42.2kPa). In addition, the elastic modulus of nucleus was 13.9kPa for hAFS cell and 26.9kPa for OB6 cells. Differences in cell elastic modulus possibly resulted from the type and number of actin cytoskeleton organization in these two cell types.
    Journal of Biomechanics 04/2013; 46(9). DOI:10.1016/j.jbiomech.2013.03.023 · 2.75 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A robust monolayer of nanoparticles is formed via dip-coating of cell culture plates. These surfaces provide cell type-specific modulation of growth behavior without the uptake of nanoparticles.
    Advanced Materials 05/2014; 26(20). DOI:10.1002/adma.201306030 · 17.49 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Highly porous 45S5 Bioglass(®)-based scaffolds with interconnected pore structure are promising candidates for bone tissue engineering due to their bioactivity, biocompatibility, osteogenic and angiogenic effects. In the present study, to ensure the mechanical competence of the 45S5 Bioglass(®)-based scaffolds, their stiffness was adjusted by applying polymer coatings and further crosslinking treatment. A non-destructive ultrasonic technique was used to determine the stiffness of the scaffolds. The stiffness of uncoated scaffolds was shown to increase by applying polymer coatings, and a further increase was achieved by crosslinking the used polymer coatings. All uncoated and polymer-coated scaffolds were confirmed to exhibit stiffness values in the range of reported values in the literature for cancellous bone. A statistical evaluation of combined multiscale ultrasound-nanoindentation measurements indicated that the stiffness of the coated scaffold is directly dependent on the stiffness of the polymer coating.
    Journal of the Mechanical Behavior of Biomedical Materials 08/2014; 40C:85-94. DOI:10.1016/j.jmbbm.2014.08.010 · 3.42 Impact Factor
Show more

Preview (2 Sources)

12 Reads
Available from