Nutrients, phytochemicals and bioactivity of wild Roman chamomile: A comparison between the herb and its preparations
ABSTRACT Roman chamomile, Chamaemelum nobile L. (Asteraceae), has been used for medicinal applications, mainly through oral dosage forms (decoctions and infusions). Herein, the nutritional characterisation of C. nobile was performed, and herbal material and its decoction and infusion were submitted to an analysis of phytochemicals and bioactivity evaluation. The antioxidant activity was determined by free radicals scavenging activity, reducing power and inhibition of lipid peroxidation, the antitumour potential was tested in human tumour cell lines (breast, lung, colon, cervical and hepatocellular carcinomas), and the hepatotoxicity was evaluated using a porcine liver primary cell culture. C. nobile proved to be an equilibrated valuable herb rich in carbohydrates and proteins, and poor in fat, providing tocopherols, carotenoids and essential fatty acids (C18:2n6 and C18:3n3). Moreover, the herb and its infusion are a source of phenolic compounds (flavonoids such as flavonols and flavones, phenolic acids and derivatives) and organic acids (oxalic, quinic, malic, citric and fumaric acids) that showed antioxidant and antitumour activities, without hepatotoxicity. The most abundant compounds in the plant extract and infusion were 5-O-caffeoylquinic acid and an apigenin derivative. These, as well as other bioactive compounds, are affected in C. nobile decoction, leading to a lower antioxidant potential and absence of antitumour potential. The plant bioactivity could be explored in the medicine, food, and cosmetic industries.
SourceAvailable from: Ângela Fernandes[Show abstract] [Hide abstract]
ABSTRACT: "Vatikiotiko" is an onion local landrace from Greece with special quality features, such as strong and pungent taste and storability. In this study, we tried for the first time to describe this landrace and record its morphological traits and nutritional value in comparison with commercially cultivated genotypes ("Creamgold", "Red Cross F1" and "Sivan F1"). Nutritional value of "Vatikiotiko" was higher than commercial genotypes, whereas total sugars, fructose and glucose content were lower in "Vatikiotiko" and "Creamgold" onions. Fatty acids composed mostly from polyunsaturated fatty acids (linoleic and α-linolenic acid), whereas for "Vatikiotiko" saturated and monounsaturated fatty acids were detected in equal amounts (29.79% and 30.60%, respectively). "Vatikiotiko" and "Creamgold" had low antioxidant activity (DPPH radical scavenging activity), especially when comparing with "Sivan F1" and "Red Cross F1", whereas no flavonoids were detected in "Vatikiotiko". The overview of "Vatikiotiko" landrace supported its special character regarding its nutritional value (sugar content, mineral composition and fatty acids profile) and the necessity to revalorize and protect its traditional culture. The potential of introducing it as Protected Designation of Origin (PDO) or Protected Geographical Indication (PGI) product has also been discussed. Copyright © 2015 Elsevier Ltd. All rights reserved.Food Chemistry 09/2015; 182. DOI:10.1016/j.foodchem.2015.03.002 · 3.26 Impact Factor
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ABSTRACT: The bioactive properties (antioxidant and antitumour activities, and hepatotoxicity) of the infusion and methanolic extracts of Chenopodium ambrosioides L., a plant commonly used in Portuguese folk medicine, were compared. The chemical composition in hydrophilic (sugars, organic acids and phenolic compounds) and lipophilic (fatty acids and tocopherols) fractions were determined. In general, the infusion revealed higher antioxidant activity, while the methanolic extract was the only one showing antitumour effects against colon, cervical and hepatocellular carcinoma cell lines. No toxicity in non-tumour cells was observed either for the infusion or the extract. The studied plant proved to be a good source of natural antioxidants and other bioactive compounds, which may have industrial use. As far as we know, this is the first detailed chemical characterization and bioactivity evaluation of C. ambrosioides methanolic extract and infusion.Journal of Functional Foods 10/2013; 5(4):1732-1740. DOI:10.1016/j.jff.2013.07.019 · 4.48 Impact Factor
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ABSTRACT: tIn this work, extracts of chamomile (Matricaria chamomilla L.) ligulate flowers obtained by Soxhlet,microwave-assisted, ultrasound-assisted and subcritical water extraction were compared in respectto their bioactivity, more specifically antioxidant, antimicrobial and cytotoxic activity. In all obtainedextracts, the content of total phenols and flavonoids was determined, indicating significant total extrac-tion yields (49.70%) and total phenol content (151.45 mg ECA/ml) in water extracts obtained undermoderate subcritical conditions. By applying DPPH and Reducing Power Test subcritical water extractionshowed superior free-radical scavenging ability in comparison to other investigated extraction tech-niques. Microbial properties of extracts were examined using eight selected indicator strains and for allextracts minimum inhibitory concentrations were in the range between 19.53 and 312.50 µ�g/ml. Subcriti-cal water extracts showed the highest activity against Escherichia coli (MIC = 39.10 �µg/ml) and Aspergillusniger (MIC = 39.10 µ�g/ml). Cytotoxic effects of extracts were examined on human rhabdomyosarcomacells (RD), cell line derived from human cervix carcinoma Hep2c (HeLa) and cell line derived from murinefibroblast (L2OB). On all three tested cell lines, subcritical water extracts demonstrated much better anti-tumor properties in comparison to extracts obtained by other techniques, with IC50values for Hep2c, RDand L2OB cells 30.54, 20.54 and 19.65 µ�g/ml, respectively.Industrial Crops and Products 10/2014; DOI:10.1016/j.indcrop.2014.09.044 · 3.21 Impact Factor