Nutrients, phytochemicals and bioactivity of wild Roman chamomile: A comparison between the herb and its preparations
Centro de Química, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.Food Chemistry (Impact Factor: 3.39). 01/2013; 136(2):718-25. DOI: 10.1016/j.foodchem.2012.08.025
Roman chamomile, Chamaemelum nobile L. (Asteraceae), has been used for medicinal applications, mainly through oral dosage forms (decoctions and infusions). Herein, the nutritional characterisation of C. nobile was performed, and herbal material and its decoction and infusion were submitted to an analysis of phytochemicals and bioactivity evaluation. The antioxidant activity was determined by free radicals scavenging activity, reducing power and inhibition of lipid peroxidation, the antitumour potential was tested in human tumour cell lines (breast, lung, colon, cervical and hepatocellular carcinomas), and the hepatotoxicity was evaluated using a porcine liver primary cell culture. C. nobile proved to be an equilibrated valuable herb rich in carbohydrates and proteins, and poor in fat, providing tocopherols, carotenoids and essential fatty acids (C18:2n6 and C18:3n3). Moreover, the herb and its infusion are a source of phenolic compounds (flavonoids such as flavonols and flavones, phenolic acids and derivatives) and organic acids (oxalic, quinic, malic, citric and fumaric acids) that showed antioxidant and antitumour activities, without hepatotoxicity. The most abundant compounds in the plant extract and infusion were 5-O-caffeoylquinic acid and an apigenin derivative. These, as well as other bioactive compounds, are affected in C. nobile decoction, leading to a lower antioxidant potential and absence of antitumour potential. The plant bioactivity could be explored in the medicine, food, and cosmetic industries.
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- "Pentagalloyl glucoside can act as a proapoptotic, anti-proliferative, anti-angiogenic and antimetastasic compound and has been used in prostate, lung, breast cancer as well as sarcoma (Zhang et al., 2009). Although the antitumor potential was not as low as ellipticine, the results were very close to the GI 50 of other natural plant extracts (Guimarães et al., 2013), and in some cases even better (Santos et al., 2013). Moreover and contrarily to ellipticine, the samples did not show any toxicity for non-tumor cells. "
ABSTRACT: Chestnut trees are one of the most important crops in the north-eastern part of Portugal, representing millions of euros of yearly income. There are many ancestral claims of the health benefits of the consumption of chestnut flowers in infusions that remain unproven. In this manuscript, the antitumor and antimicrobial potential of chestnut flowers from two cultivars, Judia and Longal, extracted through infusions and decoctions are reported. In terms of antitumor activity, the most sensitive cell lines were HepG2 and HCT15 with the cultivar Judia showing higher activity for HCT15 and Longal for HepG2, regardless of the extraction methods. Regarding the antibacterial activity of the extracts, decoctions proved to be more effective with lower minimum inhibition concentrations, while infusions were better in terms of antifungal activity. The good overall antimicrobial activity could justify the inclusion of the flowers in food chain processing to act as a natural antimicrobial. Furthermore, the results corroborate some of the ancestral claims of the consumption of these flowers.Industrial Crops and Products 12/2014; 62:42–46. DOI:10.1016/j.indcrop.2014.08.016 · 2.84 Impact Factor
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- "Sulforhodamine B assay was performed according to a procedure previously described by Guimarães et al. (2013). For hepatotoxicity evaluation, a cell culture was prepared from a freshly harvested porcine liver obtained from a local slaughter house, according to a procedure established by Guimarães et al. (2013); it was designed as PLP2. Cultivation of the cells was continued with direct monitoring every two to three days using a phase contrast microscope. "
ABSTRACT: This present investigation was undertaken to study antimicrobial and cytotoxic activities of dichloromethane, ethyl acetate, and methanol 80% extracts from Alnus rugosa L. aerial parts, as well investigate the bio-active compounds of each extract. Antimicrobial activity of the mentioned extracts against various bacteria and fungi using microdilution method, and cytotoxicity against five different human tumor cell lines, and in a non-tumor liver cells primary culture were tested. Extracts were subjected to phytochemical analysis and column chromatography eluted with different solvents, n-hexane, dichloromethane, ethyl acetate, and methanol gradually. The results showed that the extracts had a significant antimicrobial activity against all the tested bacteria and fungi, and proved a significant cytotoxic effect. Methanol 80% extract exhibited the highest antibacterial effect, and ethyl acetate extract was the most active as antifungal agent while dichloromethane extract showed the lowest antifungal effect. Dichloromethane extract was the most active against all the tested tumor cell lines. Chromatographic separation of dichloromethane extract of A. rugosa aerial parts resulted in the isolation and identification of β-sitosterol, β-sitosterol 3-O-β-glucoside and apigenin, while from ethyl acetate extract, diosmetin, naringenin, luteolin, and luteolin 7-O-β-glucoside were identified, and from methanol 80% extract, quercetin 3-O-α-rhamnoside, isorhamnetin 3-O-β-glucoside and isorhamnetin 3-O-β-glucoside 7-O-α-rhamnoside were identified. In conclusion, the present research provided significant information about antimicrobial and cytotoxic activities and compounds isolated from A. rugosa aerial parts extracts.Industrial Crops and Products 08/2014; 59:189–196. DOI:10.1016/j.indcrop.2014.05.017 · 2.84 Impact Factor
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- "Unbound SRB was removed by washing with 1% acetic acid. Plates were air-dried, the bound SRB was solubilised with 10 mM Tris (200 í µí¼L) and the absorbance was measured at 540 nm in ELX800 Microplate Reader (Bio-Tek Instruments, Inc; Winooski, USA)  "
ABSTRACT: With a complex chemical composition rich in phenolic compounds, propolis (resinous substance collected by Apis mellifera from various tree buds) exhibits a broad spectrum of biological activities. Recently, in vitro and in vivo data suggest that propolis has anticancer properties, but is the cytoxicity of propolis specific for tumor cells? To answer this question, the cytotoxicity of phenolic extracts from Portuguese propolis of different origins was evaluated using human tumor cell lines (MCF7-breast adenocarcinoma, NCI-H460-non-small cell lung carcinoma, HCT15-colon carcinoma, HeLa-cervical carcinoma, and HepG2-hepatocellular carcinoma), and non-tumor primary cells (PLP2). The studied propolis presented high cytotoxic potential for human tumor cell lines, mostly for HCT15. Nevertheless, excluding HCT15 cell line, the extracts at the GI50 obtained for tumor cell lines showed, in general, cytotoxicity for normal cells (PLP2). Propolis phenolic extracts comprise phytochemicals that should be further studied for their bioactive properties against human colon carcinoma. In the other cases, the proximity of the in vitro cytotoxic doses for tumor and normal cell lines should be confirmed by in vivo tests and may highlight the need for selection of specific compounds within the propolis extract.BioMed Research International 06/2014; 2014(2):897361. DOI:10.1155/2014/897361 · 2.71 Impact Factor
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