Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nat Nanotech 2:295-300

Nature Nanotechnology (Impact Factor: 34.05). 04/2007; 2(5):295-300. DOI: 10.1038/nnano.2007.108

ABSTRACT Surface-functionalized silica nanoparticles can deliver DNA1, 2, 3, 4, 5, 6, 7, 8 and drugs9, 10, 11, 12, 13, 14, 15 into animal cells and tissues. However, their use in plants is limited by the cell wall present in plant cells. Here we show a honeycomb mesoporous silica nanoparticle (MSN) system with 3-nm pores that can transport DNA and chemicals into isolated plant cells and intact leaves. We loaded the MSN with the gene and its chemical inducer and capped the ends with gold nanoparticles to keep the molecules from leaching out. Uncapping the gold nanoparticles released the chemicals and triggered gene expression in the plants under controlled-release conditions. Further developments such as pore enlargement and multifunctionalization of these MSNs may offer new possibilities in target-specific delivery of proteins, nucleotides and chemicals in plant biotechnology.

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    • "These NMs have served as the magic bullets for an efficient delivery system of genes (Pérez-de-Luque and Rubiales 2009). For example , Mesoporous nanosilica particles have been chemically coated and served as the gene carriers for their delivery into the tobacco and corn plants (Torney et al. 2007). These particles are absorbed through the cell wall and the target genes are efficiently delivered to the plant system in a non-toxic way. "
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    ABSTRACT: For the ever-increasing population of the world, an increasing demand for more and more food is required. To cope with this alarming situation, there is a dire need for sustainable agricultural production. In agriculture, management of optimum plant nutrients for sustainable crop production is the priority-based area of research. In this regard, much advancement in the area of plant nutrition has come forward and nano-nutrition is one the most interesting areas of research for sustainable agriculture production. Nanotechnology has revolutionized the world with tremendous advancements in many fields of science like engineering, biotechnology, analytical chemistry, and agriculture. Nano-nutrition is the application of nanotechnology for the provision of nano-sized nutrients for the crop production. Two sources of nanoparticles (NPs) have been used; biotic and abiotic. The abiotic form of nutrients or NPs is prepared from inorganic sources like salts but it is not safe because many of these are non-biodegradable. While the biotic ones are prepared from organic sources which are definitely the biodegradable and environment friendly. So, a few studies/attempts have been made in the field of nano-nutrition and a lot more are expected in the near future because this field of plant nutrition is sustainable and efficient one. Using nano-nutrition we can increase the efficiency of micro-as well as macronutrients of the plants. In this chapter, the focus has been made on the importance of nano-nutrition in the sustainable agricultural production and its future scenario so that it could be possible to apply this knowledge on a large scale without any concern regarding environment.
    • "Tsuji (2001) reported the control of parasitic weeds with properly designed functional nanocapsulated herbicides which have better penetration through cuticle to controlled release of active constituents and to reduce the phytotoxicity of herbicides on crops. Likewise, use of surface modified hydrophobic nanosilica to control a range of agricultural insect pests (Rahman et al. 2009) and surface functionalized mesoporous silica nanoparticles (MSNs) to precisely manipulate gene expression at single cell level by delivering DNA and its regulators in a controlled fashion is reported (Torney et al. 2007). Magnetic nanoparticles have shown very specific localization to release their load, which is of great interest in the study of nanoparticulate delivery for plants with no toxicity (Zhu et al. 2008). "
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    ABSTRACT: Nanotechnology requires the ability to understand the materials and precisely manipulate it to nanoscale in a useful way. Nanotechnology emerged as a new broad science of diverse fields such as basic sciences, materials science, and engineering to assemble at the nanoscale. In contrast to conventional or other contaminants, nanoparticles are posing some new environmental challenges for scientists and environmentalists worldwide. Being a new area of science, nanotechnology will leave no field untouched including agriculture and allied sectors. So far, the use of nanotechnology in agriculture has been mostly theoretical, but it has begun to have a significant effect in the main areas of agrochemical industry. Nanoparticles finding great potential as delivery systems to specific targets in living organisms and is being used in medical sciences. In plants, the same principles can be applied for a broad range of uses, particularly to tackle phytopathological infections, nutrition supplement and as growth adjuvant. Nanoparticles can be tagged to agrochemicals or other substances as delivery agent to plant system and tissues for controlled release of chemicals. Doing so, the negative effects of nanomaterials on plant productivity and soil microbes and environment must not be overlooked, such as toxicity generated by free radicals leading to lipid peroxidation and DNA damage. Key focus of the chapter particularly relates the use of nanoparticles on agricultural crops and its toxic implications to plants and microbes naturally present in soil and generation of nanowaste in agroecosystem.
    Nanotechnology and Plant Sciences: Nanoparticles and Their Impact on Plants, M.H. Siddiqui et al. (eds.) edited by Manzer H. Siddiqui, Mohamed H. Al-Whaibi, Mohammad Firoz, 01/2015: chapter Implications of Nanotechnology on Plant Productivity and Its Rhizospheric Environment: pages 37-53; Springer International Publishing, Switzerland., ISBN: 978-3-319-14502-0
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    • "Transgenic technology has attracted the attention of scientists in various fields, including medicine [1]–[4], agriculture [5]–[8] and biology [9]–[12]. Gene delivery, the process of introducing foreign DNA into host cells, is a necessary step in the genetic modification of crops and livestock. "
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    ABSTRACT: Superparamagnetic nanoparticles are promising candidates for gene delivery into mammalian somatic cells and may be useful for reproductive cloning using the somatic cell nuclear transfer technique. However, limited investigations of their potential applications in animal genetics and breeding, particularly multiple-gene delivery by magnetofection, have been performed. Here, we developed a stable, targetable and convenient system for delivering multiple genes into the nuclei of porcine somatic cells using magnetic Fe3O4 nanoparticles as gene carriers. After surface modification by polyethylenimine, the spherical magnetic Fe3O4 nanoparticles showed strong binding affinity for DNA plasmids expressing the genes encoding a green (DNAGFP) or red (DNADsRed) fluorescent protein. At weight ratios of DNAGFP or DNADsRed to magnetic nanoparticles lower than or equal to 10∶1 or 5∶1, respectively, the DNA molecules were completely bound by the magnetic nanoparticles. Atomic force microscopy analyses confirmed binding of the spherical magnetic nanoparticles to stretched DNA strands up to several hundred nanometers in length. As a result, stable and efficient co-expression of GFP and DsRed in porcine kidney PK-15 cells was achieved by magnetofection. The results presented here demonstrate the potential application of magnetic nanoparticles as an attractive delivery system for animal genetics and breeding studies.
    PLoS ONE 07/2014; 9(7):e102886. DOI:10.1371/journal.pone.0102886 · 3.23 Impact Factor
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