Article

Formation of artificial lipid bilayers using droplet dielectrophoresis

School of Electronics and Computer Science, University of Southampton, Southampton, UK.
Lab on a Chip (Impact Factor: 5.75). 11/2008; 8(10):1617-20. DOI: 10.1039/b807374k
Source: PubMed

ABSTRACT We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid-alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.

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