Article

A highly sensitive and selective diagnostic assay based on virus nanoparticles

Department of Chemical and Biological Engineering, Korea University, Anam-Dong 5-1, Seongbuk-Gu, Seoul 136-713, Republic of Korea.
Nature Nanotechnology (Impact Factor: 33.27). 03/2009; 4(4):259-264. DOI: 10.1038/nnano.2009.38

ABSTRACT Early detection of the protein marker troponin I in patients with a higher risk of acute myocardial infarction1, 2, 3, 4, 5 can reduce the risk of death from heart attacks6, 7, 8, 9, 10. Most troponin assays are currently based on the conventional enzyme linked immunosorbent assay and have detection limits in the nano- and picomolar range11. Here, we show that by combining viral nanoparticles, which are engineered to have dual affinity for troponin antibodies and nickel, with three-dimensional nanostructures including nickel nanohairs, we can detect troponin levels in human serum samples that are six to seven orders of magnitude lower than those detectable using conventional enzyme linked immunosorbent assays11, 12, 13, 14, 15, 16. The viral nanoparticle helps to orient the antibodies for maximum capture of the troponin markers. High densities of antibodies on the surfaces of the nanoparticles and nanohairs lead to greater binding of the troponin markers, which significantly enhances detection sensitivities. The nickel nanohairs are re-useable and can reproducibly differentiate healthy serum from unhealthy ones. We expect other viral nanoparticles to form similar highly sensitive diagnostic assays for a variety of other protein markers.

Download full-text

Full-text

Available from: Young Keun Kim, Sep 20, 2014
2 Followers
 · 
175 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Here a novel technique is reported to construct a three-dimensional (3D) array of well-defined and controllable multilayered nanostructures of proteins that is based on alternate layer-by-layer assembly of bacterial protein nanoparticles and DNA on a patterned array of gold dots. This is the first report on protein-based multilayer stacking, which has the following significant advantages over conventional multilayer assemblies: 1) avoiding hazardous chemicals, the multilayer assembly is implemented in aqueous solution under mild temperature and pH conditions over a relatively short period; 2) direct multilayer growth from designated position is possible by controlling the aspect ratio; 3) multicomponent stacking can be easily performed through alternate stacking of different building blocks (in this case protein nanoparticles); and 4) a wide variety of 3D arrays can be constructed using various functionalized protein nanoparticles that are easily prepared through a simple genetic engineering approach. In this study, as a proof of concept, the developed 3D and patterned arrays of protein nanoparticle multilayers are successfully applied to the multiplexed bioassays of breast and colorectal cancer markers.
    Advanced Functional Materials 12/2010; 20(23):4055 - 4061. DOI:10.1002/adfm.201001144 · 10.44 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: PGCS-NPs (40 nm) with excellent photo-thermal activity are developed, on the surface of which affibody peptides with specific affinity for EGFR and many small gold dots (1-3 nm) are densely presented. The IV-injected PGCS-NPs into EGFR-expressing tumor-bearing mice successfully perform targeted and photothermal therapy of cancer. It seems that the small gold dots released from disassembled PGCS-NPs are easily removed and never cause in vivo toxicity problems.
    Advanced Materials 10/2014; 26(37). DOI:10.1002/adma.201401499 · 15.41 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Filamentous bacteriophages have successfully been used to detect chemical and biological analytes with increased selectivity and sensitivity. The enhancement largely originates not only from the ability of viruses to provide a platform for the surface display of a wide range of biological ligands, but also from the geometric morphologies of the viruses that constitute biomimetic structures with larger surface area-to-volume ratio. This review will appraise the mechanism of multivalent display of the viruses that enables surface modification of virions either by chemical or biological methods. The accommodation of functionalized virions to various materials, including polymers, proteins, metals, nanoparticles, and electrodes for sensor applications will also be discussed.
    Sensors 08/2014; 14(8):13592-612. DOI:10.3390/s140813592 · 2.05 Impact Factor