Domain specific genetic mosaic system in the Drosophila eye

Department of Biology, University of Dayton, Dayton, OH 45469, USA.
genesis (Impact Factor: 2.04). 01/2013; 51(1). DOI: 10.1002/dvg.22355
Source: PubMed

ABSTRACT Genetic mosaic approach is commonly employed in the Drosophila eye by completely abolishing or misexpressing a gene within a subset of cells to unravel its role during development. Classical genetic mosaic approach involves random clone generation in all developing fields. Consequently, a large sample size needs to be screened to generate and analyze clones in specific domains of the developing eye. To address domain specific functions of genes during axial patterning, we have developed a system for generating mosaic clones by combining Gal4/UAS and FLP/FRT system which will allow generation of loss-of-function as well as gain-of-function clones on the dorsal and ventral eye margins. We used the bifid-Gal4 driver to drive expression of UAS-flippase (FLP). This reagent can have multiple applications in (i) studying spatio-temporal function of a gene during dorso-ventral axis specification in the eye, (ii) analyzing genetic epistasis of genes involved in DV patterning and (iii) conducting genome wide screens in a domain specific manner. © 2012 Wiley Periodicals, Inc.

Download full-text


Available from: Amit Singh, Aug 06, 2015
  • Source
    • "results in pigmentation on the dorsal and ventral eye margins in the adult eye . An omb - Gal4 line that was later isolated by Calleja and colleagues and Tang and Sun has been used vastly as an important tool to drive expression of genes on the dorsal and ventral borders margins of the eye imaginal disc ( Calleja et al . 1996 ; Tang and Sun 2002 ; Tare et al . 2013 ) ."
    Molecular Genetics of Axial Patterning, Growth and Disease in the Drosophila Eye, Edited by Amit Singh, Madhuri Kango-Singh, 10/2013: chapter Molecular Genetic Mechanisms of Axial Patterning: Mechanistic Insights into Generation of Axes in the Developing Eye: pages 37-73; Springer NewYork., ISBN: ISBN 978-1-4614-8232-1