Domain specific genetic mosaic system in the Drosophila eye

Department of Biology, University of Dayton, Dayton, OH 45469, USA.
genesis (Impact Factor: 2.02). 01/2013; 51(1). DOI: 10.1002/dvg.22355
Source: PubMed


Genetic mosaic approach is commonly employed in the Drosophila eye by completely abolishing or misexpressing a gene within a subset of cells to unravel its role during development. Classical genetic mosaic approach involves random clone generation in all developing fields. Consequently, a large sample size needs to be screened to generate and analyze clones in specific domains of the developing eye. To address domain specific functions of genes during axial patterning, we have developed a system for generating mosaic clones by combining Gal4/UAS and FLP/FRT system which will allow generation of loss-of-function as well as gain-of-function clones on the dorsal and ventral eye margins. We used the bifid-Gal4 driver to drive expression of UAS-flippase (FLP). This reagent can have multiple applications in (i) studying spatio-temporal function of a gene during dorso-ventral axis specification in the eye, (ii) analyzing genetic epistasis of genes involved in DV patterning and (iii) conducting genome wide screens in a domain specific manner. © 2012 Wiley Periodicals, Inc.

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    • "results in pigmentation on the dorsal and ventral eye margins in the adult eye . An omb - Gal4 line that was later isolated by Calleja and colleagues and Tang and Sun has been used vastly as an important tool to drive expression of genes on the dorsal and ventral borders margins of the eye imaginal disc ( Calleja et al . 1996 ; Tang and Sun 2002 ; Tare et al . 2013 ) ."
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    ABSTRACT: All multicellular organisms require axial patterning to transform a single-layer organ primordium to a three-dimensional organ. It involves delineation of anteroposterior (AP), dorsoventral (DV), and proximodistal (PD) axes. Any deviation in this fundamental process results in patterning and growth defects during organogenesis. The Drosophila eye is an excellent model to study axial patterning. In the Drosophila eye, DV lineage is the first axis to be determined, which is followed by generation of the AP axis. The default state of the Drosophila early eye primordium is ventral, and the dorsal fate is established by onset of expression of dorsal eye fate selector pannier (pnr)in a group of cells on the dorsal eye margin. The boundary between dorsal and ventral compartments is the site for activation of Notch (N) signaling and is referred to as the equator. Activation of N signaling is crucial for initiating the cell proliferation and differentiation in the developing Drosophila eye imaginal disc. This chapter will focus on (a) how axial patterning occurs in the developing Drosophila eye, (b) how the developing eye field gets divided into dorsal and ventral cell populations, and (c) how DV patterning genes contribute toward the growth and patterning of the fly retina. © 2013 Springer Science+Business Media New York. All rights are reserved.
    Molecular Genetics of Axial Patterning, Growth and Disease in the Drosophila Eye, Edited by Amit Singh, Madhuri Kango-Singh, 10/2013: chapter Molecular Genetic Mechanisms of Axial Patterning: Mechanistic Insights into Generation of Axes in the Developing Eye: pages 37-73; Springer NewYork., ISBN: ISBN 978-1-4614-8232-1