Article

Conformational signatures of 13C chemical shifts in RNA ribose.

Leibniz Institute for Age Research/Fritz Lipmann Institute, Biomolecular NMR Spectroscopy, Beutenbergstrasse 11, Jena, Germany.
Journal of Biomolecular NMR (impact factor: 3.61). 10/2008; 42(2):139-42. DOI:10.1007/s10858-008-9271-1 pp.139-42
Source: PubMed

ABSTRACT The conformational dependence of (13)C chemical shift values of RNA riboses determined by liquid-state NMR spectroscopy was evaluated using data deposited for RNA structures in the RCSD and BMRB data bases. Results derived support the applicability of the canonical coordinates approach of Rossi and Harbison (J Magn Reson 151:1-8, 2001) in liquid-state NMR to assess the sugar pucker of ribose units in RNA.

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    Article: DNA structures from phosphate chemical shifts.
    Joséphine Abi-Ghanem, Brahim Heddi, Nicolas Foloppe, Brigitte Hartmann
    [show abstract] [hide abstract]
    ABSTRACT: For B-DNA, the strong linear correlation observed by nuclear magnetic resonance (NMR) between the (31)P chemical shifts (deltaP) and three recurrent internucleotide distances demonstrates the tight coupling between phosphate motions and helicoidal parameters. It allows to translate deltaP into distance restraints directly exploitable in structural refinement. It even provides a new method for refining DNA oligomers with restraints exclusively inferred from deltaP. Combined with molecular dynamics in explicit solvent, these restraints lead to a structural and dynamical view of the DNA as detailed as that obtained with conventional and more extensive restraints. Tests with the Jun-Fos oligomer show that this deltaP-based strategy can provide a simple and straightforward method to capture DNA properties in solution, from routine NMR experiments on unlabeled samples.
    Nucleic Acids Research 11/2009; 38(3):e18. · 8.03 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

13)C chemical shift values
 
applicability
 
conformational dependence
 
liquid-state NMR
 
liquid-state NMR spectroscopy
 
RCSD
 
ribose units
 
RNA
 
RNA riboses
 
RNA structures
 
sugar pucker
 

Oliver Ohlenschläger