Article

Proteomic detection of changes in protein expression induced by cordycepin in human hepatocellular carcinoma BEL-7402 cells.

Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China.
Methods and Findings in Experimental and Clinical Pharmacology (impact factor: 0.93). 07/2008; 30(5):347-53. DOI:10.1358/mf.2008.30.5.1186085 pp.347-53
Source: PubMed

ABSTRACT The nucleoside analogue cordycepin (3 '-deoxyadenosine, 3 '-dA), one of the components of Cordyceps militaris, has been shown to inhibit the growth of various tumor cells. However, the probable mechanism is still obscure. In this study, the inhibition of cell growth and changes in protein expression induced by cordycepin were investigated in BEL-7402 cells. Using the MTT assay and flow cytometry, we found that cordycepin inhibits cell viability and induces apoptosis in BEL-7402 cells. Additionally, the proteins were separated using two-dimensional polyacrylamide gel electrophoresis, and eight proteins were found to be significantly affected by cordycepin compared to untreated control; among them, two were downregulated and six were upregulated. Of the eight proteins, six were identified with peptide mass fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) after in-gel trypsin digestion. These proteins are involved in various aspects of cellular metabolism. It is suggested that the effect of cordycepin on the growth of tumor cells is significantly related to the metabolism-associated protein expression induced by cordycepin.

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Keywords

cellular metabolism
 
cordycepin
 
cordycepin inhibits cell viability
 
eight proteins
 
flow cytometry
 
in-gel trypsin digestion
 
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
 
metabolism-associated protein expression induced
 
nucleoside analogue cordycepin
 
peptide mass fingerprinting
 
probable mechanism
 
proteins
 
two-dimensional polyacrylamide gel electrophoresis
 
untreated control
 
various aspects
 
various tumor cells
 

Ping Shi