Best practices of ASRM and ESHRE: a journey through reproductive medicine
ABSTRACT BACKGROUND: The American Society for Reproductive Medicine (ASRM) and the European Society of Human Reproduction and Embryology (ESHRE) are the two largest societies in the world whose members comprise the major experts and professionals working in the field of reproductive medicine and embryology. These societies have never before had a joint scientific meeting. METHODS: A 3-day meeting was planned and took place in March of 2012. The goal was to present and debate key topics, as well as modes of practice in reproductive medicine and to discuss recent developments in the field. RESULTS: Presentations by members of ASRM and ESHRE were of three types: 'state of the art' lectures, 'back-to-back' presentations of two points of view and debates. CONCLUSIONS: For the first time, ASRM and ESHRE held a joint meeting where a special emphasis was given to presentations on the hottest topics in the field. Although different opinions and approaches sometimes exist on the two sides of the Atlantic, an appreciation and acceptance of these differences was evident, and there was more commonality than divergence of opinion.
- SourceAvailable from: Adel Helal[Show abstract] [Hide abstract]
ABSTRACT: The aim of this study was to examine the effect of clomiphene citrate [CC] co-administration during the use of exogenous low-dose urinary FSH [uFSH] for induction of ovulation in CC-resistant infertile PCOS women. In a randomised controlled setting, 174 CC-resistant infertile PCOS women were randomized into two parallel groups; Group I received CC 100 mg/day for 5 days plus uFSH 37.5 IU/day while group II received only uFSH 37.5 IU /day. Subsequent increments of uFSH by 37.5 IU/day were made according to response. Primary outcome was ovulation rate. Secondary outcomes were clinical pregnancy rates, number of follicles, endometrial thickness, and gonadotropins consumption. Our results have demonstrated that group I compared to group II had significantly higher ovulation rate per intention to treat [ITT] [72.4 % vs. 34.2 %, p < 0.001]. Clinical pregnancy and live birth rates were comparable between the two groups. Group I consumed significantly lower total FSH dose and needed significantly shorter stimulation duration compared to group II. CC co-administered during low dose HP uFSH versus uFSH for CC-resistant PCOS yields significantly higher ovulation rate and less consumption of FSH.Journal of Assisted Reproduction and Genetics 09/2013; 30(11). DOI:10.1007/s10815-013-0090-2 · 1.77 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Nanomaterial-mediated delivery represents a promising technique for reproductive biology with a potential to improve the safety and efficacy of existing methodologies, including experimental gene therapy and sperm-mediated gene transfer. Mesoporous silica nanoparticles (MSNPs) have been characterised as a powerful and safe delivery tool, rendering them an excellent candidate for use in reproductive research. However, their effects upon mammalian gametes with highly specialised structure and functionality remain untested. Here, we show for the first time, that spherical MSNPs with hexagonal pore symmetry, functionalised with polyethileneimine and aminopropyltriethoxysilane, and optionally loaded with two common types of cargo (nucleic acid/protein), form strong associations with boar sperm following incubation in vitro and do not exert negative effect upon the main parameters of sperm function, including motility, viability, acrosomal status and DNA fragmentation index. Our findings provide a rationale for the use of MSNPs for the transfer of investigative, diagnostic and/or therapeutic compounds into mammalian sperm.Nanomedicine: nanotechnology, biology, and medicine 11/2013; 10(4):859-70. DOI:10.1016/j.nano.2013.10.011 · 5.98 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Introduction the embryo biopsy aims to select genetically normal embryos. This selection occurs through pre‐ implantation genetic testing. It is expected the reduction of risk of genetic disorders and increase implantation rates in IVF. Objective to verify, through bibliographical revision, which embryo biopsy technique is considered more suitable for pre‐implantation genetic diagnosis. Method bibliographical research, in the form of literary review of scientific publications via networks, US National Library of Medicine (Pubmed), Latin‐American Literature and Caribbean Health Sciences (Lilacs), Google Scholar and Virtual Health Library. Results and conclusion there are three ways to perform the biopsy on assisted human reproduction. The first one consists in removing the 1st and/or 2nd polar body (if there was fertilization). You can also perform the biopsy from the one blastomere of embryo cleavage stage or use 5‐10 trophoectoderm cells blastocyst. Usually the techniques used for diagnostic purpose are PCR, Fish, CGH array, SNP array and others. Nowadays it is believed that blastocyst biopsy is the best technique in order to maintain the embryonic implantation. This tendency is justified by the larger amount of genetic material available in an advanced stage of embryonic development. It is assumed that in this stage the incidence of mosaicism is reduced with the consequent increase in the effectiveness of genetic testing. Another important question is that the blastocyst biopsy cells are removed from the trophoectoderm while inbiopsy incleavage stage, the removal of oneblastomerecan impairembryonic development.01/2014; 28(3). DOI:10.1016/j.recli.2014.06.001