Article

Validation of the body fluid module on the new Sysmex XN-1000 for counting blood cells in cerebrospinal fluid and other body fluids

Clinical Chemistry and Laboratory Medicine (Impact Factor: 2.96). 10/2012; 50(10):1791-8. DOI: 10.1515/cclm-2011-0927
Source: PubMed

ABSTRACT Abstract Background: We evaluated the body fluid (BF) module on the new Sysmex XN-1000 for counting blood cells. Methods: One hundred and eighty-seven BF samples [73 cerebrospinal fluid (CSF), 48 continuous ambulatory peritoneal dialysis (CAPD), 46 ascites, and 20 pleural fluid] were used for method comparison between the XN-1000 and manual microscopy (Fuchs-Rosenthal chamber and stained cytospin slides) for counting red blood cells (RBCs) and white blood cells (WBCs) (differential). Results: Good agreement was found for counting WBCs (y=1.06x+0.09, n=67, R2=0.96) and mononuclear cells (MNs) (y=1.04x-0.01, n=40, R2=0.93) in CSF. However, the XN-1000 systematically counted more polymorphonuclear cells (PMNs) (y=1.48x+0.18, n=40, R2=0.99) compared to manual microscopy. Excellent correlation for RBCs >1×109/L (y=0.99x+116.56, n=26, R2=0.99) in CSF was found. For other fluids (CAPD, ascites and pleural fluid) excellent agreement was found for counting WBCs (y=1.06x+0.26, n=109, R2=0.98), MNs (y=1.06x-0.41, n=93, R2=0.96), PMNs (y=1.06x+0.81, n=93, R2=0.98) and RBCs (y=1.04x+110.04, n=43, R2=0.98). By using BF XN-check, the lower limit of quantitation (LLoQ) for WBC was defined at 5×106/L. Linearity was excellent for both the WBCs (R2=0.99) and RBCs (R2=0.99) and carry-over never exceeded 0.05%. Conclusions: The BF module on the XN-1000 is a suitable tool for fast and accurate quantification of WBC (differential) and RBC counts in CSF and other BFs in a diagnostic setting.

Full-text

Available from: Robert de Jonge, May 30, 2015
23 Followers
 · 
1,935 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: INTRODUZIONE Il liquor o liquido cefalorachidiano (LCR) è un materiale che la modalità di prelievo rende "unico". La sua peculiarità di essere a stretto contatto con lo spazio extravascolare del sistema nervoso centrale (SNC) e isolato dagli altri liquidi corporei lo rende indispensabile per la diagnostica di molte patologie del SNC. Nonostante l'esistenza di linee guida generali e specifiche per i vari argomenti, l'esame del LCR risente di un approccio troppo spesso non standardizzato che, attraverso una gestione confusa e frammentata, può ripercuotersi sull'esito clinico. Questo documento, preparato dal Gruppo di Studio SIBioC -Medicina di Laboratorio "Biochimica clinica dei liquidi biologici non ematici", è nato con l'intento di dare indicazioni di corretto comportamento per la gestione dell'analisi liquorale, desunte dalla letteratura esistente e sostenute dall'esperienza in questo campo specifico. Nel documento si sottolinea l'importanza di tutte le fasi delle indagini liquorali, che impongono una preparazione culturale specifica e un addestramento adeguato degli operatori. Vengono date indicazioni sulle fasi preanalitiche, analitiche e postanalitiche dell'indagine e affrontati i principali quesiti diagnostici per i quali è richiesta l'analisi liquorale. Il documento non affronta nella sua interezza e complessità il tema delle indagini microbiologiche, che in un secondo tempo sarà L'analisi del liquido cefalorachidiano
    Biochimica clinica 08/2014; 38(3):238-254.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Introduction The Sysmex XN-series is a new automated hematology analyzer designed to improve the accuracy of cell counts and the specificity of the flagging events.Methods The basic characteristics and the performance of new measurement channels of the XN were evaluated and compared with the Sysmex XE-2100 and the manual method. Fluorescent platelet count (PLT-F) was compared with the flow cytometric method. The low WBC mode and body fluid mode were also evaluated. For workflow analysis, 1005 samples were analyzed on both the XN and the XE-2100, and manual review rates were compared.ResultsAll parameters measured by the XN correlated well with the XE-2100. PLT-F showed better correlation with the flow cytometric method (r2 = 0.80) compared with optical platelet count (r2 = 0.73) for platelet counts <70 × 109/L. The low WBC mode reported accurate leukocyte differentials for samples with a WBC count <0.5 × 109/L. Relatively good correlation was found for WBC counts between the manual method and the body fluid mode (r = 0.88). The XN made less flags than the XE-2100, while the sensitivities of both instruments were comparable.Conclusion The XN provided reliable results on low cell counts, as well as reduced manual blood film reviews, while maintaining a proper level of diagnostic sensitivity.
    International journal of laboratory hematology 05/2014; 37(2). DOI:10.1111/ijlh.12254 · 1.87 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Automated cell counting for body fluids is gradually replacing manual cell counting by hemocytometer. Automation offers potential benefits of improved accuracy, efficiency, and standardization. The addition of body fluid modes to some hematology analyzers adapts the technology and software to meet the particular requirements of body fluid analysis. However, the functional sensitivity for low cell counts currently limits applicability of automated methods to all types of body fluid. Microscopic review is indicated when malignancy is a diagnostic consideration. Copyright © 2015 Elsevier Inc. All rights reserved.
    Clinics in Laboratory Medicine 03/2015; 35(1):93-103. DOI:10.1016/j.cll.2014.10.003 · 1.35 Impact Factor