Listeriosis: a primer.

Department of Pediatrics, IWK Health Centre and Dalhousie University, Halifax, NS.
Canadian Medical Association Journal (Impact Factor: 5.81). 10/2008; 179(8):795-7. DOI: 10.1503/cmaj.081377
Source: PubMed
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    ABSTRACT: Listeria species may cause life-threatening events including meningitis and invasive infection in newborns, pregnant women, older and immunodeficient people. The most common Listeria species that causes infection is L. monocytogenes. It is known that Listeria innocua has no pathogenicity. A 9-month-old baby had ventriculoperitoneal shunt and was treated with adrenocorticotropic hormone because of infantile spasms. He was brought to hospital with fever and vomiting. Upon physical examination, the patient seemed uncomfortable and had a temperature of 38.6°C. Laboratory results were as follows: hemoglobin, 6.7 g/dL; leukocyte count, 5420/mm3; platelet count, 169 000/mm3; and C-reactive protein, 100 mg/L (normal <5 mg/L). On analysis of cerebrospinal fluid (CSF), leukocyte count was 480/mm3, protein was 46 mg/dL and CSF glucose was 35 mg/dL. L. innocua was isolated in CSF culture. We describe this unusual case of ventriculoperitoneal shunt infection with L. innocua.
    Pediatrics International 08/2014; 56(4). DOI:10.1111/ped.12302 · 0.73 Impact Factor
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    ABSTRACT: Bacteriophages and bacteriocins are promising biocontrol tools in food. In this work, two Listeria bacteriophages, FWLLm1 and FWLLm3, were assessed in combination with the bacteriocin coagulin C23 to inhibit Listeria monocytogenes. Preliminary results under laboratory conditions demonstrated that both antimicrobials act synergistically when they were applied in suboptimal concentrations. The combined approach was further assessed in milk contaminated with 5×10(4)CFU/ml L. monocytogenes 2000/47 and stored at 4°C for 10days. When used alone, phage FWLLm1 added at 5×10(6)PFU/ml, FWLLm3 at 5×10(5)PFU/ml and coagulin C23 at 584AU/ml kept L. monocytogenes 2000/47 counts lower than the untreated control throughout storage. However, when used in combination, inhibition was enhanced and in the presence of FWLLm1 and coagulin C23, L. monocytogenes 2000/47 counts were under the detection limits (less than 10CFU/ml) from day 4 until the end of the experiment. Resistant mutants towards phages and coagulin C23 could be obtained, but cross-resistance was not detected. Mutants resistant to FWLLm3 and coagulin C23 were also recovered from surviving colonies after cold storage in milk which may explain the failure of this combination to inhibit L. monocytogenes. Remarkably, the fraction of resistant mutants isolated from the combined treatment was lower than that from each antimicrobial alone, suggesting that synergy between bacteriocins and phages could be due to a lower rate of resistance development and the absence of cross-resistance. Copyright © 2015 Elsevier B.V. All rights reserved.
    International Journal of Food Microbiology 07/2015; 205:68-72. DOI:10.1016/j.ijfoodmicro.2015.04.007 · 3.16 Impact Factor
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