Transcriptional Silencing by Single-Stranded RNAs Targeting a Noncoding RNA That Overlaps a Gene Promoter

Departments of Pharmacology and Biochemistry, UT Southwestern Medical Center , 6001 Forest Park Road, Dallas, Texas 75390-9041, United States.
ACS Chemical Biology (Impact Factor: 5.36). 10/2012; 8(1). DOI: 10.1021/cb300490j
Source: PubMed

ABSTRACT RNAi using single-strand RNA would provide new options for therapeutic development and for investigating critical questions of mechanism. Using chemically modified single-strands, we test the hypothesis that single-stranded RNAs can engage the RNAi pathway and silence gene transcription. We find that a chemically modified single-stranded silencing RNA (ss-siRNA) designed to be complementary to a long noncoding RNA (lncRNA) requires argonaute protein, functions through the RNAi pathway, and inhibits gene transcription. These data expand the use of single-stranded RNA to cell nuclei.

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