Phenotype and function of human T lymphocyte subsets: Consensus and issues

Cellular Immunology Laboratory, INSERM U543, Avenir Group, Hôpital Pitié-Salpêtrière, Université Pierre et Marie Curie-Paris6, 75013 Paris, France.
Cytometry Part A (Impact Factor: 3.07). 11/2008; 73(11):975-83. DOI: 10.1002/cyto.a.20643
Source: PubMed

ABSTRACT In recent years, a tremendous effort has been devoted to the detailed characterization of the phenotype and function of distinct T cell subpopulations in humans, as well as to their pathway(s) of differentiation and role in immune responses. But these studies seem to have generated more questions than definitive answers. To clarify issues related to the function and differentiation of T cell subsets, one session of the MASIR 2008 conference was dedicated to this topic. Several points of consensus and discord were highlighted in the work presented during this session. We provide here an account of these points, including the relative heterogeneity of T cell subpopulations during infections with distinct pathogens, the relationship between phenotypic and functional T cell attributes, and the pathway(s) of T cell differentiation. Finally, we discuss the problems which still limit general agreement.

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    • "Following antigenic recognition, autoreactive CD8+ T cells undergo clonal expansion and differentiate into different subsets, including central-memory (T CM ), effector-memory (T EM ) and effector (T EFF ) cells characterized by changes in extracellular receptors such as, the isoform of common lymphocyte antigen, CD45RA, chemokine receptor CCR7 (CD197) and intracellular expression of effector molecules [12] [13]. The pattern of differentiation is mainly determined by the strength and duration of antigenic signal and requires aid of CD4+ T cells [14] [15] [16] [17]. Hence, besides frequency, determination of phenotype of autoreactive CD8+ T cells also becomes important in monitoring disease progression in T1D subjects or individuals at risk and assessing efficacy of therapeutic or transplant approaches [18] [19]. "
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    ABSTRACT: Latent autoimmune diabetes in adults (LADA) resembles type 1 diabetes (T1D) in disease presentation except that its onset is slow. We compared pathophysiological characteristics of CD8 + T cells recognizing preproinsulin (PPI) derived epitopes in both disease groups using MHC-I dextramers (DMRs) in peripheral blood and after in-vitro stimulation with PPI. Subjects with T1D harboured higher frequency of DMR + CD8 + T cells with relatively higher frequency of effector T cell subsets. Following stimulation with PPI, an increase in DMR + CD8 + T cells, particularly the central-memory subset was observed in T1D group, whereas no significant change in DMR + CD8 + T cell subsets was observed in LADA group. Intracellular expression of Granzyme-B and Perforin in DMR + CD8 + T cells was comparable in both the groups. In conclusion, lower frequency and inferior proliferative potential on account of a relatively restrained central-memory subset of PPI specific CD8 + T cells are associated with slow rate of disease progression in LADA.
    Clinical Immunology 01/2015; 157(1). DOI:10.1016/j.clim.2015.01.005 · 3.99 Impact Factor
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    • "It seems plausible that the subpopulations that are more metabolically and inflammation-active will have higher expression, i.e. CD16+ monocytes and the effector memory and senescent CD45RA+ effector memory CD8+ T cell subpopulations (Appay et al., 2008; Ziegler-Heitbrock et al., 2010). Of particular note, responses in CD3+ T cells did not fully account for the expression of GHS-R1a observed in total lymphocytes. "
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    Brain Behavior and Immunity 10/2013; 39. DOI:10.1016/j.bbi.2013.09.017 · 6.13 Impact Factor
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    • "After this initial response, the infection activates the adaptive immunity process: dendritic cells other than participating to the innate inflammatory response have the ability to capture and present antigens to B and T cells of the acquired immune system [28]. Activated CD4 T-helper cells produce subsets of cytokines which will define phenotypically distinguished immune responses: Th-1 and Th-2 cells, respectively, associate with cellular and humoral immunity [29] and the recently described Th-17 and T regulatory (Treg) cells, which have antagonistic roles as effector and suppressive cells, respectively [10] [30] [31]. B cells are also activated and are transformed into plasma cells, which produce antibodies against bacterial antigens. "
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    Clinical and Developmental Immunology 05/2013; 2013:503754. DOI:10.1155/2013/503754 · 2.93 Impact Factor
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