A Novel Synthesis of Chitosan Nanoparticles in Reverse Emulsion

BioMerieux, Chemin de l'orme, Marcy l'Etoile 69280, France.
Langmuir (Impact Factor: 4.46). 10/2008; 24(20):11370-7. DOI: 10.1021/la801917a
Source: PubMed


Physical hydrogels of chitosan in the colloidal domain were obtained in the absence of both cross-linker and toxic organic solvent. The approach was based on a reverse emulsion of a chitosan solution in a Miglyol/Span 80 mixture, generally regarded as safe. Temperature and surfactant concentration were optimized, and the impact of the degree of acetylation (DA) and the molar mass of chitosan was investigated. When chitosan had a DA above 30%, only macroscopic gels were obtained, because of the predominance of attractive Van der Waals forces. The lower the molar mass of chitosan, the better the control over particle size and size distribution, probably as a result of either a reduction in the viscosity of the internal aqueous phase or an increase in the disentanglement of the polymer chain during the process. After extraction and redispersion of the colloid in an ammonium acetate buffer, the composition of the particles was around 80% of pure chitosan corresponding to a recovery of 60% of the original input. These new and safe colloids offer wide perspectives of development in further applications.

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Available from: Laurent Veron, Oct 05, 2015
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    • "ement with the report by Jang et al . ( 2008 ) that showed the particle size of the Chitosan - Ascorbic acid nanoparticles decreased with increasing heat treatment . According to the Eötvös empirical equation , the general trend is that the surface tension decreases with increasing temperature , reaching a value of zero at a critical temperature ( Brunel et al . , 2008 ) . This could explain why the particle size decreased when temperature increased from 40 o C to 50 o C . Therefore , particle size is important to enhance the nanoparticles mediated bioactive compounds of liquid smoke , such as phenolic should be entrapped in the smallest CS - MD nanoparticles by using heat treatment at 50 o C and conc"
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    ABSTRACT: The study investigated the characteristics of chitosan-maltodextrin (CS-MD) nanoparticles incorporating coconut shell liquid smoke at various formulations. Chitosan-maltodextrin nanoparticles were prepared with the addition of 1.0% sodium tripolyphosphate (TPP) into a solution of liquid smoke. Sample consisting of CS-MD nanoparticles in 1.0% acetic acid without liquid smoke was used as a control. CS-MD nanoparticles were also evaluated at elevated temperatures (40 and 50 o C) for 15 min. The CS-MD nanoparticles with the liquid smoke resulted in the range of pH from 2.41 to 3.02; viscosity 10.83 cP -11.77 cP; particle size 1.3 nm -12.7 nm and the zeta potential (-6.53) mV – (+3.12) mV. While, the control CS-MD nanoparicles without coconut shell liquid smoke showed pH 3.09; viscosity 11.17 cP; particle size 343.86 nm and the zeta potential (+5.17) mV.
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    • "More water should be added if nanoparticles of a larger size are to be prepared. Brunel et al. (2008) used a reverse micellar method to prepare chitosan nanoparticles. The lower the molar mass of chitosan, the better the control over particle size and size distribution, probably as a result of either a reduction in the viscosity of the internal aqueous phase or an increase in the disentanglement of the polymer chains during the process. "
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    ABSTRACT: Encapsulation and immobilization technology is important for the food processing and bioengineering industries. Chitosan is a natural polysaccharide prepared by the N-deacetylation of chitin. It has been widely used in food and bioengineering industries, including the encapsulation of active food ingredients, in enzyme immobilization, and as a carrier for controlled drug delivery, due to its significant biological and chemical properties such as biodegradability, biocompatibility, bioactivity, and polycationicity. In this work, chitosan nanoparticles and nanofibers used to encapsulate bioactive substances and immobilize enzymes were reviewed. Preparation of chitosan nanoparticles and nanofibers, including the work achieved in our group on chitosan nanoparticles for enzyme immobilization, were also introduced. Some problems encountered with nano-structured chitosan carriers for bioactive substance encapsulation and enzyme immobilization were discussed, together with the future prospects of such systems.
    Brazilian Journal of Chemical Engineering 09/2011; 28(3):353-362. DOI:10.1590/S0104-66322011000300001 · 1.04 Impact Factor
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    ABSTRACT: In this research, chitosan nanoparticles were prepared based on the ionic gelation of chitosan with tripolyphosphate anions. Effects of parameters such as chitosan concentration, tripolyphosphate concentration, and reaction time on the particle size distribution were investigated. In order to determine optimum conditions, tests were designed by Qualitek-4 software, using Taguchi method. The best conditions were determined based on three factors at three levels. Therefore, the main object was to investigate the effect of some parameters on particle size distribution and determine the optimum conditions for preparing chitosan nanoparticles by ionic gelation, followed by evaluation of the physicochemical and structural properties. The size distribution of original chitosan and chitosan nanoparticles were determined by Laser Diffraction and Dynamic Light Scattering, respectively. The physicochemical properties of the chitosan nanoparticles were studied using SEM, TEM, XRD pattern, FTIR, UV–vis, TGA, and NMR spectra. The optimum chitosan concentration, tripolyphosphate concentration, and reaction time were found to be 1.0 mg/ml, 1.0 mg/ml, and 60 min, respectively.
    Journal of Cluster Science 09/2013; 24(3). DOI:10.1007/s10876-013-0583-2 · 1.30 Impact Factor
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