Indirect immunofluorescence as a diagnostic tool for parvovirus infection of broiler chickens.
ABSTRACT Nuclear fluorescence was seen in the epithelial cells of the duodenum of broiler chicks infected experimentally at 1-day-old with a parvovirus of chicken origin. No antigenic relationship was detected by this method between chicken and goose parvoviruses.
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- "EM examination of intestinal contents should be done after CsCl-gradient ultracentrifugation to eliminate most other small round viruses, and this method should be accompanied by biochemical studies aimed at characterizing the viral genome (Kisary et al., 1984). A simple, rapid FA procedure has been described, but this procedure requires specific antiserum that is not widely available (Kisary, 1985b). A parvovirus-like virus of turkeys was described by Trambel et al. (1982). "
ABSTRACT: Several different viruses have been identified as causes of gastrointestinal tract infections in poultry. These include rotaviruses, coronaviruses, enteroviruses, adenoviruses, astroviruses, and reoviruses. In addition, a number of other viruses of unknown importance have been associated with gastrointestinal diseases in poultry based on electron microscopic examination of feces and intestinal contents. Viral infections of the gastrointestinal tract of poultry are known to negatively impact poultry production, and they likely contribute to the development of other, extragastrointestinal diseases. Our current understanding of the viruses that cause gastrointestinal tract infections in poultry is reviewed, with emphasis given to those of greatest importance.Poultry Science 09/1998; 77(8):1166-75. DOI:10.1093/ps/77.8.1166 · 1.67 Impact Factor
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ABSTRACT: Chicken embryo fibroblast cell cultures prepared from commercial uninoculated White Leghorn eggs showed a spontaneous degeneration. Upon staining with haematoxylin-eosin intranuclear inclusion bodies of Cowdry type A were seen. Also, nuclear fluorescence was detected after indirect immunofluorescence staining with anti-serum to fowl parvovirus strain ABU. Electron microscopy of thin sections revealed parvovirus-like particles in both the nucleus and cytoplasm of the affected cells. During purification the viral particles banded in CsCl at a density of 1.42 to 1.43 g/ml and displayed typical parvovirus morphology by negative-staining EM. The viral particles contained single-stranded DNA of one polarity with a natural primer at the 3'-end of the molecules. All these properties are characteristic of self-replicating fowl parvovirus strongly suggesting its transovarial transmission.Avian Pathology 02/1987; 16(1):115-21. DOI:10.1080/03079458708436357 · 2.04 Impact Factor
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ABSTRACT: An indirect immunofluorescence (IIF) test for the detection of lympho-proliferative disease virus (LPDV) is described. Results presented show that smears of buffy coat cells and frozen sections of spleen, but not thymus, form suitable test materials for a rapid and specific diagnosis of LPDV infection of turkeys. Between 0.1 and 0.6% of buffy coat cells from infected birds were antigen positive, and spleens from 18/18 and thymuses from 11/18 birds respectively, tested 16 months after viral inoculation, were positive. Antisera containing group-specific antibodies to avian reticuloendotheliosis virus (REV) and avian sarcoma-leukosis virus (ASLV) did not cross-react with LPDV-infected buffy coat cells or spleens.Avian Pathology 02/1987; 16(3):367-76. DOI:10.1080/03079458708436387 · 2.04 Impact Factor