A red-emitting naphthofluorescein-based fluorescent probe for selective detection of hydrogen peroxide in living cells.

Aaron E Albers, Bryan C Dickinson, Evan W Miller, Christopher J Chang

Department of Chemistry, University of California, 532A Latimer Hall, Berkeley, CA 94720, USA.

Journal Article: Bioorganic & medicinal chemistry letters (impact factor: 2.65). 08/2008; DOI: 10.1016/j.bmcl.2008.08.035

Abstract

We report the synthesis, properties, and cellular application of Naphtho-Peroxyfluor-1 (NPF1), a new fluorescent indicator for hydrogen peroxide based on a red-emitting naphthofluorescein platform. Owing to its boronate cages, NPF1 features high selectivity for hydrogen peroxide over a panel of biologically relevant reactive oxygen species (ROS), including superoxide and nitric oxide, as well as excitation and emission profiles in the far-red region of the visible spectrum (>600nm). Flow cytometry experiments in RAW264.7 macrophages establish that NPF1 can report changes in peroxide levels in living cells.

Source: PubMed

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Keywords

biologically relevant reactive oxygen species
 
cellular application
 
emission profiles
 
excitation
 
far-red region
 
Flow cytometry experiments
 
hydrogen peroxide
 
new fluorescent indicator
 
visible spectrum