Preparation of chorionic villus samples for metaphase chromosome analysis and chromosomal microarray analysis
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas.Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.] 10/2012; Chapter 8:Unit8.3. DOI: 10.1002/0471142905.hg0803s75
Chorionic villi are composed of an outer layer of trophoblastic cells and an inner mesenchymal cell core. They can be prepared for chromosome analysis using a culture method wherein villi are disaggregated by mechanical and enzymatic methods and the resulting cell suspension is used to establish primary cultures. Mesenchymal cells of the villus core are released by this procedure and the fibroblasts are actively proliferative in tissue culture. Cultures can be used for cytogenetic analysis after ∼1 week. Slides prepared by this technique can be stained using trypsin-Giemsa banding and analyzed for chromosomal abnormalities in fetal tissue. Chorionic villi may also be assessed by chromosomal microarray analysis (CMA). For this purpose, a method for extraction of high-quality DNA from CVS tissue is also described here. Curr. Protoc. Hum. Genet. 75:8.3.1-8.3.9. © 2012 by John Wiley & Sons, Inc.
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ABSTRACT: From the experience accumulated in less than 4 years of chorionic villus sampling activity, much has been learned about the advantages of prenatal diagnosis in the first trimester. The safety of the procedure during this developmental stage has been extremely good. The record in this centre with over 2700 cases sampled and over 2000 deliveries has demonstrated a fetal loss rate similar to that observed in amniocentesis studies. It appears that fetal loss that may be attributed to the procedure is not more than 1% and may be less. Some of that loss appears to be correlated with avoidable trauma to the chorionic membrane area. If this conclusion is valid, then avoidance of this pattern even in the occasional difficult case may further improve the already acceptable safety record of the procedure.Baillière s Clinical Obstetrics and Gynaecology 10/1987; 1(3-1):513-531. DOI:10.1016/S0950-3552(87)80004-4
- Akusherstvo i ginekologiia 09/1979;
- Orvosi Hetilap 12/1980; 121(45):2765-6.
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