Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor
ABSTRACT We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd(2+) bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd(2+). CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd(2+) levels with the use of two excited wavelengths.
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- "Therefore development of reliable chemo-sensors for detection and monitoring of cadmium in environment and biology is still in demand. However, numerous numbers of fluorescent sensors have been reported to detect and analyze different kinds of heavy toxic metal ions because of their simplicity, high sensitivity, and real-time detection   but there are only a few reports of fluorescent sensors for cadmium ion      , and even rare examples are available in living cells      . The paramount challenge in the detection of Cd 2+ is the interference of other transition metal ions, in particular Zn 2+ , which have similar properties to those of Cd 2+ because they are in the same group of the periodic table. "
ABSTRACT: A novel simple C3v-symmetric, completely water soluble quinoline based guanidinium Schiff's base system have been reported for the selective detection of Cd2+ in physiological environment through a turn-on fluorescence response. The cadmium complex is found to be highly selective toward iodide by turning off the fluorescence through the sequestration of Cd2+ from L1-Cd by the precipitation of CdI2. Detection limit for Cd2+ and iodide are found to be 51 ppb and 1 ppb, respectively. L1 is reversible toward Cd2+ and iodide and can be recycled. The receptor was used for the detection of Cd2+ in complex natural samples (tap, lake and river water).Sensors and Actuators B Chemical 12/2014; 204:474. DOI:10.1016/j.snb.2014.08.004 · 4.29 Impact Factor
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ABSTRACT: A highly selective and sensitive OFF-ON fluorescent sensor 1, employing the PET mechanism, was designed and synthesized. It could be used to detect Cd(2+) ion in aqueous solution and to image Cd(2+) ion in living cells. The fluorescence intensity significantly enhanced about 195-fold and the quantum yield increased almost 100-fold. Moreover the fluorescence intensity of 1 increased linearly with high sensitivity (0-1 microM) toward Cd(2+).Journal of the American Chemical Society 12/2008; 130(48):16160-1. DOI:10.1021/ja806928n · 11.44 Impact Factor
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ABSTRACT: A fluorescent sensor, QA, based on acetamidoquinoline with DPA as receptor, was synthesized. In aqueous buffer solution, QA demonstrates high selectivity for sensing Cd(2+) with about 40-fold enhancement in fluorescence quantum yield and picomolar sensitivity (K(d) = 0.25 +/- 0.03 pM). Moreover, sensor QA can distinguish Cd(2+) from Zn(2+) via two different sensing mechanisms (PET for Cd(2+); ICT for Zn(2+)), and the discrimination is even possible by "naked eyes".Organic Letters 05/2009; 11(7):1655-8. DOI:10.1021/ol900315r · 6.32 Impact Factor