Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor

Graduate School of Human and Environmental Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.
Journal of the American Chemical Society (Impact Factor: 11.44). 09/2008; 130(38):12564-5. DOI: 10.1021/ja803429z
Source: PubMed

ABSTRACT We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd(2+) bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd(2+). CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd(2+) levels with the use of two excited wavelengths.

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