A GAL4-Driver Line Resource for Drosophila Neurobiology

Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
Cell Reports (Impact Factor: 8.36). 10/2012; 2(4). DOI: 10.1016/j.celrep.2012.09.011
Source: PubMed

ABSTRACT We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.

93 Reads
  • Source
    • "An intersectional screen identifies fru M + second-and third-order auditory neurons Motivated by the hypothesis that fru M labels neurons that detect courtship-relevant sensory stimuli (Manoli et al., 2005; Stockinger et al., 2005), we performed an anatomical screen aimed at identifying fru M + neurons in the auditory pathway. Specifically, ∼1000 cis regulatory module (CRM) GAL4 lines with relatively sparse neuronal expression patterns (Jenett et al., 2012) were crossed to LexAop2-FLP; fru LexA , UAS>stop>myr::GFP to restrict expression of GFP to those neurons that express both GAL4 and fru LexA (Figure 1J). These intersectional expression patterns were then registered onto a standard brain for analysis of potentially overlapping projection patterns. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Animals use acoustic signals across a variety of social behaviors, particularly courtship. In Drosophila, song is detected by antennal mechanosensory neurons and further processed by second-order aPN1/aLN(al) neurons. However, little is known about the central pathways mediating courtship hearing. In this study, we identified a male-specific pathway for courtship hearing via third-order ventrolateral protocerebrum Projection Neuron 1 (vPN1) neurons and fourth-order pC1 neurons. Genetic inactivation of vPN1 or pC1 disrupts song-induced male-chaining behavior. Calcium imaging reveals that vPN1 responds preferentially to pulse song with long inter-pulse intervals (IPIs), while pC1 responses to pulse song closely match the behavioral chaining responses at different IPIs. Moreover, genetic activation of either vPN1 or pC1 induced courtship chaining, mimicking the behavioral response to song. These results outline the aPN1-vPN1-pC1 pathway as a labeled line for the processing and transformation of courtship song in males.
    eLife Sciences 09/2015; 4. DOI:10.7554/eLife.08477 · 9.32 Impact Factor
  • Source
    • "This system allows in vivo repurposing of gene expression patterns through genetic crossing schemes to switch between binary systems (Gal4, LexA, Q) or to achieve intersection by introducing Gal80 or Split-Gal4 hemi-drivers (Gohl et al. 2011) the minimal number of cells required for a behavior may be inhibited by the lack of enhancer-trap expression patterns with sufficiently restricted patterns to be informative for mapping. It may require the combination of a collection of enhancer-trap or promoter-driven Gal4 lines and ET-FLP lines to produce smaller intersection patterns (Pfeiffer et al. 2008; Jenett et al. 2012). Third, the number of ET-FLPx2 lines currently available most likely limits the power of the FINGR system. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Understanding behavior requires unraveling the mysteries of neurons, glia, and their extensive connectivity. Drosophila has emerged as an excellent organism for studying the neural basis of behavior. This can be largely attributed to the extensive effort of the fly community to develop numerous sophisticated genetic tools for visualizing, mapping, and manipulating behavioral circuits. Here, we attempt to highlight some of the new reagents, techniques and approaches available for dissecting behavioral circuits in Drosophila. We focus on detailing intersectional strategies such as the Flippase-induced intersectional Gal80/Gal4 repression (FINGR), because of the tremendous potential they possess for mapping the minimal number of cells required for a particular behavior. The logic and strategies outlined in this review should have broad applications for other genetic model organisms.
    Journal of Comparative Physiology 04/2015; 201(9). DOI:10.1007/s00359-015-1010-y · 2.04 Impact Factor
  • Source
    • "The presynaptic terminals from 0104-labeled dopaminergic neurons densely innervate the b 0 and g lobe tips of the horizontal mushroom body lobes, which suggests that appetitive olfactory memories may be represented as changes in the efficacy of synaptic outputs in these regions from the odor-activated KCs onto as-yet-unidentified downstream neurons. By visually screening available GAL4 collections (Jenett et al., 2012; Bidaye et al., 2014), we identified three fly lines that labeled candidate postsynaptic neurons with arbors in the tip regions, b 2 , b 0 2 , and g 5 , of the horizontal mushroom body lobes (Figure 1). Neurons innervating b 0 2 and g 5 have been described as MB-M4 and MB-M6 (Tanaka et al., 2008). "
    [Show abstract] [Hide abstract]
    ABSTRACT: During olfactory learning in fruit flies, dopaminergic neurons assign value to odor representations in the mushroom body Kenyon cells. Here we identify a class of downstream glutamatergic mushroom body output neurons (MBONs) called M4/6, or MBON-β2β'2a, MBON-β'2mp, and MBON-γ5β'2a, whose dendritic fields overlap with dopaminergic neuron projections in the tips of the β, β', and γ lobes. This anatomy and their odor tuning suggests that M4/6 neurons pool odor-driven Kenyon cell synaptic outputs. Like that of mushroom body neurons, M4/6 output is required for expression of appetitive and aversive memory performance. Moreover, appetitive and aversive olfactory conditioning bidirectionally alters the relative odor-drive of M4β' neurons (MBON-β'2mp). Direct block of M4/6 neurons in naive flies mimics appetitive conditioning, being sufficient to convert odor-driven avoidance into approach, while optogenetically activating these neurons induces avoidance behavior. We therefore propose that drive to the M4/6 neurons reflects odor-directed behavioral choice. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
    Neuron 04/2015; 86(2). DOI:10.1016/j.neuron.2015.03.025 · 15.05 Impact Factor
Show more