A GAL4-Driver Line Resource for Drosophila Neurobiology

Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
Cell Reports (Impact Factor: 8.36). 10/2012; 2(4). DOI: 10.1016/j.celrep.2012.09.011
Source: PubMed


We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.

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Article: A GAL4-Driver Line Resource for Drosophila Neurobiology

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    • "The presynaptic terminals of PAM-α1 are localized to the basal compartment of the MB α lobe (α1) (Liu et al., 2012), whereas the dendrites stretch horizontally across the superior medial, intermediate, and lateral protocerebra (SMP, SIP, and SLP, respectively) (Aso et al., 2014a; Yamagata et al., 2015) (Figure 1A,B,H). By segmenting the dendrites of PAM-α1 in confocal images (Aso et al., 2014a), we queried a database of GAL4 expression patterns (Jenett et al., 2012) to computationally search for candidate upstream neurons. Strikingly, one of the input candidates we identified was the output neurons from the α1 compartment of the MB (MBON-α1) (Figure 1C,D). "
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