Protective role of Beta vulgaris L. leaves extract and fractions on ethanol-mediated hepatic toxicity
ABSTRACT The present study was undertaken to investigate the hepatoprotective action of Beta vulgaris Linn. (BV, Chenopodiaceae) leaves against ethanol-mediated hepatotoxicity. Rat hepatocyte culture and rats were used as in vitro and in vivo screening models, respectively. In the in vitro studies, different extracts (i.e., petroleum ether, ethanol and aqueous) and fractions derived from ethanol extract (i.e., chloroform, ethyl acetate and n-butanol) of BV leaves were screened. In vivo hepatoprotective activity was assessed in rats intoxicated with ethanol. Levels of serum markers enzymes together with antioxidants were measured to evaluate the extent of hepatic protection. Silymarin was taken as reference drug. In the in vitro studies, n-butanol fraction of BV leaves (BVBF) was found to be more potent than others. Moreover, in the in vivo evaluation, BVBF at doses of 50, 100 and 200 mg/kg showed marked protective action against ethanol-induced hepatic toxicity as evident by restoration of biochemical changes caused by ethanol. The present study concluded that BVBF possess potent hepatoprotective effect against ethanol-induced hepatic toxicity and it may have a great potential role in the management of alcoholic liver disease.
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ABSTRACT: The present study was undertaken to investigate the antihepatotoxic activity of ethanolic extract, aqueous extract and compound isolated from the ethanolic extract of the barks of Crataeva nurvala (CN) against CCl4 induced hepatotoxicity. Alteration in the levels of biochemical markers of hepatic damage like SGOT, SGPT, SALP and bilirubin were tested in vivo in both CCl4 treated and untreated groups using albino rats. In vitro determination of SGPT and LDH was also carried out using isolated rat hepatocytes. Isolation of compound from the ethanolic extract and its structural elucidation was done simultaneously. From the spectroscopic observations using UV, FTIR, NMR and GCMS, isolated compound was found to be lupeol. Treatment with ethanolic extract (250 and 500 mg/kg p.o.) and isolated lupeol (50 mg/kg p.o.) altered the levels of biochemical markers to almost normal in a dose dependent manner as compared to aqueous extract (250 and 500 mg/kg p.o.). Our findings suggested that CN ethanolic bark extract and the isolated compound (lupeol) possessed antihepatotoxic activity.06/2014; 4(2):101-110. DOI:10.1080/22311866.2013.868705
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ABSTRACT: Liver disease is one of the major causes of morbidity and mortality across the world. According to WHO estimates, about 500 million people are living with chronic hepatitis infections resulting in the death of over one million people annually. Medicinal plants serve as a vital source of potentially useful new compounds for the development of effective therapy to combat liver problems. Moreover herbal products have the advantage of better affordability and acceptability, better compatibility with the human body, and minimal side effects and is easier to store. In this review attempt has been made to summarize the scientific data published on hepatoprotective plants used in Saudi Arabian traditional medicine. The information includes medicinal uses of the plants, distribution in Saudi Arabia, ethnopharmacological profile, possible mechanism of action, chemical constituents, and toxicity data. Comprehensive scientific studies on safety and efficacy of these plants can revitalise the treatment of liver diseases.Evidence-based Complementary and Alternative Medicine 12/2014; 2014:890842. DOI:10.1155/2014/890842 · 1.88 Impact Factor