Article

Markers of Antigen Presentation and Activation on Eosinophils and T-Cells in the Esophageal Tissue of Patients with Eosinophilic Esophagitis.

*Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Stanford University Medical Center, Lucile Packard Children's Hospital, Palo Alto, CA †Department of Pediatrics, Division of Allergy and Immunology, Stanford University Medical Center, Lucile Packard Children's Hospital, Palo Alto, CA ‡Pediatric Center for Inflammatory Bowel Disorders, California Pacific Medical Center, San Francisco, CA.
Journal of pediatric gastroenterology and nutrition (Impact Factor: 2.87). 10/2012; DOI: 10.1097/MPG.0b013e3182758d49
Source: PubMed

ABSTRACT OBJECTIVES:: Evidence suggests eosinophils may be acting as antigen presenting cells (APC) by presenting antigen to T-cells. We investigated the surface proteins of eosinophils and T-cells in the esophageal biopsies of eosinophilic eosphagitis (EoE) patients, gastroeosphageal reflux (GERD) patients, and healthy controls. METHODS:: Subjects were categorized as EoE, GERD or healthy control (HC). In esophageal tissue, EG2+ eosinophils were stained for the antigen presenting cell markers, CD40 or CD80, via immunohistochemistry. CD3+ T-cells were stained for co-stimulatory markers CD40L or CD28, and for activation markers, CD69 or CD134 via immunofluorescence or immunohistochemistry. RESULTS:: Eosinophils stained with CD40 and CD80. The number of EG2+CD40+ cells was increased in EoE (mean = 19.1 ± 14.8 cells/HPF, n = 11), in comparison to GERD (mean = 0.13 ± 0.19 cells/HPF, n = 5, p < 0.01) and HC (mean = 0.3 ± 0.7 cells/HPF, n = 5, p < 0.01). There was an elevation in EG2+CD80+ cells in EoE (mean = 18.1 ± 16.2 cells/HPF, n = 10), GERD (mean = 1.7 ± 2.8 cells/HPF, n = 6, p < 0.01) or HC (mean = 0.8 ± 1.3 cells/HPF, n = 6, p < 0.01). CD3+ T-cells stained with CD40L (not quantified). CD3+ T-cells stained with CD28 at elevated levels in EoE (mean = 14 ± 8.7 cells/HPF, n = 9), versus GERD (mean = 3.3 ± 1.2 cells/HPF, n = 6, p < 0.05) or HC (mean = 3.0 ± 3.2 cells/HPF, n = 7, p < 0.01). The number of CD3+CD69+ cells was highest in EoE (mean = 14.8 ± 7.5 cells/HPF, n = 6) versus GERD (mean = 0.8 ± 0.9 cells/HPF, n = 6, p < 0.001) or HC (mean = 2.7 ± 2.5 cells/HPF, n = 6, p < 0.001). CONCLUSIONS:: We show that esophageal eosinophils express CD40 and CD80, and T-cells with CD40L, CD28 and CD69. The number of double stained cells was higher in EoE in comparison to controls groups. These data support the hypothesis that eosinophils in EoE might act as APC, activating T-cells.

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