Cribriform adenocarcinoma of minor salivary glands may express galectin-3, cytokeratin 19, and HBME-1 and contains polymorphisms of RET and H-RAS proto-oncogenes

The Fingerland Department of Pathology, Charles University Faculty of Medicine and Faculty Hospital, Sokolska 581, 500 05, Hradec Králové, Czech Republic, .
Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin (Impact Factor: 2.65). 09/2012; 461(5). DOI: 10.1007/s00428-012-1320-1
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The aim of the study was to further elucidate the immunohistochemical and genetic characteristics of cribriform adenocarcinoma of minor salivary glands (CAMSG). The study comprised five CAMSG from two males and three females, aged 21-72 years. Four tumors were localized at the base of tongue and one in the floor of mouth. At the time of diagnosis, four tumors had metastasised to regional lymph nodes. After tumor resection, two patients were treated by radiotherapy and one by chemoradiotherapy. During the follow-up (median 14 months), two patients developed lymph node metastasis. Microscopically, all tumors showed cribriform, papillary, follicular, and microcystic growth patterns. The tumor cells displayed vesicular nuclei with intranuclear grooves. Immunohistochemically, all tumors showed expression of cytokeratin (CK) 7, CK8, CK18, vimentin, smooth muscle actin, calponin, S-100 protein, and p16 protein. In addition, we observed expression of galectin-3, CK19, and HBME-1, but not of thyroglobulin and TTF-1. No mutations of RET, BRAF, K-RAS, H-RAS, and N-RAS proto-oncogenes were detected. However, in RET proto-oncogene, we found polymorphisms Gly691Ser (exon 11) and Ser904Ser (exon 15) in one case, p.Leu769Leu (exon 13) in one case, and variant p.IVS14-24 G/A of intron 14 in two cases, and in H-RAS proto-oncogene we found polymorphism 81 T-C (exon 1) in three cases. Thyroglobulin and TTF-1 are the only useful markers in the differential diagnosis between CAMSG and papillary thyroid carcinoma as both tumors may express galectin-3, CK19, and HBME-1. The RET, H-RAS, and N-RAS proto-oncoogenes are not mutated in CAMSG.

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    • "Therefore, we investigated whether ethanol exposure alters specific proteins and/or induces apoptosis in the parenchyma glandular cells. It is well noticed that previous immunohistochemical studies that assess atrophy in salivar glands are related to identification the diagnosis, or in experimental conditions, are caused by ductal ligation as noxious stimulus (Burgess et al., 1996; Burgess and Dardick, 1998; Takahashi et al., 1998; Safadi et al., 2010; Laco et al., 2012). Thereby, our study emerges as a relevant work that highlight the immunohistochemical evaluation in salivary glands after damage produced by chronic chemical stimulus. "
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