Article

Diversity of viruses detected by deep sequencing in pigs from a common background

Virus and Prion (Cheung, Lager) and Infectious Bacterial Diseases (Alt, Bayles) Research Units, National Animal Disease Center, U.S. Department of Agriculture, Agricultural Research Service, Ames, IA.
Journal of veterinary diagnostic investigation: official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (Impact Factor: 1.23). 10/2012; 24(6). DOI: 10.1177/1040638712463212
Source: PubMed

ABSTRACT Although advances in nucleic acid sequencing have enabled the discovery of many infectious agents, challenges remain for scientists and veterinary diagnosticians trying to design animal studies with a minimum of variables and to interpret laboratory results. To evaluate pyrosequencing technology as a potential screening method to estimate the virome in pigs, fecal samples were collected from 4 pigs out of a group of 175 that had been raised together since birth. A number of viruses were detected, demonstrating the application of this technology to determine the background "noise" in the pigs. However, pyrosequencing also demonstrated the diversity of viruses within a group of animals and how that can confound experimental design and obscure a definitive diagnosis.

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