Antifungal effect of silver nanoparticles on dermatophytes
ABSTRACT Spherical silver nanoparticles (nano-Ag) were synthesized and their antifungal effects on fungal pathogens of the skin were investigated. Nano-Ag showed potent activity against clinical isolates and ATCC strains of Trichophyton mentagrophytes and Candida species (IC80, 1-7 microg/ml). The activity of nano-Ag was comparable to that of amphotericin B, but superior to that of fluconazole (amphotericin B IC80, 1-5 microg/ml; fluconazole IC80, 10- 30 microg/ml). Additionally, we investigated their effects on the dimorphism of Candida albicans. The results showed nano-Ag exerted activity on the mycelia. Thus, the present study indicates nano-Ag may have considerable antifungal activity, deserving further investigation for clinical applications.
Full-textDOI: · Available from: Keuk-Jun Kim, Nov 13, 2014
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- "SNPs at 100 ppm totally inhibited the bacterial growth, but the activity against mold and dermatophytes was low; bacteria and molds exhibited resistance against SNPs at 50 ppm concentration (Falkiewicz-Dulik and macura et al. 2008). In case of C.albicans also, SNPs are found to be cytotoxic disrupting the cell membrane and formation of pits and pores on membrane surface, subsequently leads to cell death (Kim et al. 2008, 2009). SNPs have found its application against fungal infection and in biostabilization of footwear materials. "
ABSTRACT: Silver ions (Ag+) and its compounds are highly toxic to microorganisms, exhibiting strong biocidal effects on many species of bacteria but have a low toxicity toward animal cells. In the present study, silver nanoparticles (SNPs) were biosynthesized using aqueous extract of Chlorella vulgaris as reducing agent and size of SNPs synthesized ranged between 15 and 47 nm. SNPs were characterized by UV-visible spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction and Fourier infrared spectroscopy, and analyzed for its antibacterial property against human pathogens. This approach of SNPs synthesis involving green chemistry process can be considered for the large-scale production of SNPs and in the development of biomedicines.03/2015; DOI:10.1007/s13204-015-0426-6
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- "ranging from 1 to 100 nm. Silver nanoparticles represent a prominent nanoproduct with potential applications in medicine and hygiene because of the antibacterial effects (Lok et al., 2006; Kim et al., 2007; Ayala-Núñez et al., 2009) antiviral actions (Elechiguerra et al., 2005; Mehrbod et al., 2009) and antifungal activity (Kim et al., 2008a). They also promote wound healing by playing a role in cytokine modulation (Wong et al., 2009). "
ABSTRACT: Silver nanoparticles (AgNPs) have wide medical applications regarding their antimicrobial effects. They are applied also in appliances such as refrigerators and washing machines. For assessment of toxicological potential of silver nanoparticles 20 mature female albino rats were divided into four groups (five rats per each). Animals were injected i/p by different doses of approximately 8.7 nm silver nanoparticles (1, 2 and 4 mg/kg b.w) daily for 28 days in addition to control group which were injected by deionized water only. Indicators of oxidative stress in liver tissue, determination of silver nanoparticles tissue concentration, description of hepatic histopathological alterations and detection of possible chromosomal aberrations in bone marrow were carried out. Results revealed various hepatic histopathological lesions that were dose dependent. The effect of Ag-NPs on hepatic malondialdhyde (MDA) and glutathione (GSH) levels were variable in different treated groups compared with the control. The tissue residues of silver nanoparticles were found in hepatic tissue and related to original treated dose. Finally, silver nanoparticles induced variable chromosomal aberrations that were dose dependent. Conclusion: Silver nanoparticles had the ability for inducing various hepatic histopathological alterations indicating hepatocytotoxicity presumably by oxidative stress, in addition to the induction of chromosomal aberrations in bone marrow cells denoting the genotoxicity of nanosilver particles.Experimental and Toxicologic Pathology 01/2015; 67(1). DOI:10.1016/j.etp.2014.09.005 · 2.01 Impact Factor
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- "According with the definition of fungistatic versus fungicidal effect , in our study AgNPs were found to present a fungicidal effect, since cultures treated with the MIC were unable to recover after treatment, even after inoculation in fresh culture medium in successive subcultures. The antifungal properties of AgNPs against C. albicans have been demonstrated in some other studies, although reported MIC values are different from the ones we found in this work , , , , , , . Such differences could be due to the nature of the particles used, the difference in size being particularly important. "
ABSTRACT: Candida albicans is the most common fungal pathogen in humans, and recently some studies have reported the antifungal activity of silver nanoparticles (AgNPs) against some Candida species. However, ultrastructural analyses on the interaction of AgNPs with these microorganisms have not been reported. In this work we evaluated the effect of AgNPs on C. albicans, and the minimum inhibitory concentration (MIC) was found to have a fungicidal effect. The IC50 was also determined, and the use of AgNPs with fluconazole (FLC), a fungistatic drug, reduced cell proliferation. In order to understand how AgNPs interact with living cells, the ultrastructural distribution of AgNPs in this fungus was determined. Transmission electron microscopy (TEM) analysis revealed a high accumulation of AgNPs outside the cells but also smaller nanoparticles (NPs) localized throughout the cytoplasm. Energy dispersive spectroscopy (EDS) analysis confirmed the presence of intracellular silver. From our results it is assumed that AgNPs used in this study do not penetrate the cell, but instead release silver ions that infiltrate into the cell leading to the formation of NPs through reduction by organic compounds present in the cell wall and cytoplasm.PLoS ONE 10/2014; 9(10):e108876. DOI:10.1371/journal.pone.0108876 · 3.23 Impact Factor