Article

Detection of Disordered Regions in Globular Proteins Using 13C-Detected NMR

Department of Pathology, University of Michigan, 1150 W. Medical Center Drive, MSRB1 4516, Ann Arbor, MI 48109
Protein Science (Impact Factor: 2.86). 12/2012; 21(12). DOI: 10.1002/pro.2174

ABSTRACT Characterization of disordered regions in globular proteins constitutes a significant challenge. Here, we report an approach based on 13C-detected NMR experiments for the identification and assignment of disordered regions in large proteins. Using this method we demonstrate that disordered fragments can be accurately identified in two homologs of menin, a globular protein with a molecular weight over 50 kDa. Our work provides an efficient way to characterize disordered fragments in globular proteins for structural biology applications.

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Available from: Marcelo J Murai, Mar 30, 2015
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