Proportions of Mycobacterium massiliense and Mycobacterium bolletii Strains among Korean Mycobacterium chelonae-Mycobacterium abscessus Group Isolates

Department of Microbiology, Cancer Research Institute, SNUMRC, Seoul National University College of Medicine, and Seoul National University Hospital, Seoul 110-799, Republic of Korea.
Journal of clinical microbiology (Impact Factor: 3.99). 09/2008; 46(10):3384-90. DOI: 10.1128/JCM.00319-08
Source: PubMed


Korean isolates of the Mycobacterium chelonae-Mycobacterium abscessus group, which had been isolated from two different hospitals in South Korea, were identified by PCR restriction analysis (PRA)
and comparative sequence analysis of 16S rRNA genes, rpoB, and hsp65 to evaluate the proportion of four closely related species (M. chelonae, M. abscessus, M. massiliense, and M. bolletii). Of the 144 rapidly growing mycobacterial strains tested, 127 strains (88.2%) belonged to the M. chelonae-M. abscessus group. In this group, M. chelonae, M. abscessus, M. massiliense, and M. bolletii accounted for 0.8% (n = 1), 51.2% (n = 65), 46.5% (n = 59), and 1.6% (n = 2), respectively. Two isolates which showed discordant results, M. massiliense by rpoB sequence analysis and M. abscessus by hsp65 sequence analysis, were finally identified as M. massiliense based on the additional analysis of sodA and the 16S-23S internal transcribed spacer. M. abscessus group I isolates previously identified by hsp65 PRA were all found to be M. abscessus, whereas group II isolates were further identified as M. massiliense or M. bolletii by sequencing of rpoB and hsp65. Smooth, rough, or mixed colonies of both M. abscessus and M. massiliense isolates were observed. M. massiliense strains that were highly resistant to clarithromycin had a point mutation at the adenine at position 2058 (A2058) or 2059 (A2059) in the peptidyltransferase region of the 23S rRNA gene.

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Available from: Bum-Joon Kim, Oct 13, 2015
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    • "M. abscessus complex (MABC) is the second most common etiologic organism (14-33%) of NTM lung disease in Korea17,18,19,24. Within MABC, M. abscessus (44-53%) and M. massiliense (45-55%) are equally distributed, while M. bolletii is a relatively rare pathogen (1-2%) in Korea25,26,27. In addition, M. kansasii is infrequently isolated and a relatively uncommon cause of NTM lung disease in Korea28,29. "
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    ABSTRACT: The recovery of nontuberculous mycobacteria (NTM) from respiratory specimens and the number of patients with NTM lung disease have been rapidly increasing in Korea. An early differential diagnosis of NTM lung disease from pulmonary tuberculosis (TB) is important, as the therapeutic regimen differs from that of pulmonary TB, and it is not necessary to track the contacts of patients with NTM lung disease. However, differentiating NTM lung disease from pulmonary TB remains difficult, because the clinical presentations of the two diseases are similar and a definite diagnosis of NTM lung disease based on sputum culture takes time. This review focuses on the changing epidemiology, clinical and radiographic manifestation, and laboratory diagnosis of pulmonary TB and NTM lung disease in Korea.
    Tuberculosis and Respiratory Diseases 07/2014; 77(1):1-5. DOI:10.4046/trd.2014.77.1.1
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    • "However, interspecific relationships have been identified within the M. abscessus group through genotype analysis, such as PCR restriction analysis (PRA) and sequencing of hsp65 and rpoB. Moreover, it has been recently reported that M. massiliense and M. bolletii are very closely related to M. abscessus [10-12, 19, 20]. In the present study M. abscessus and M. massiliense strains were differentiated on the basis of the erm(41) gene, and M. abscessus and M. bolletii were differentiated utilizing the -35 sequence difference in erm(41) gene promoter. "
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    ABSTRACT: Bacteria of the Mycobacterium abscessus group are the second most common pathogens responsible for lung disease caused by nontuberculous mycobacteria in Korea. There is still a lack of studies investigating the genetic mechanisms involved in M. abscessus resistance to antibiotics other than clarithromycin. This study investigated the characteristics of drug resistance exhibited by M. abscessus clinical isolates from Korea. We performed drug susceptibility testing for a total of 404 M. abscessus clinical strains. Subspecies were differentiated by molecular biological methods and examined for mutations in drug resistance-related genes. Of the 404 strains examined, 202 (50.00%), 199 (49.26%), and 3 (0.74%) strains were identified as M. abscessus, M. massiliense, and M. bolletii, respectively. Of the 152 clarithromycin-resistant strains, 6 possessed rrl mutations, while 4 of the 30 amikacin-resistant strains contained rrs mutations, and 5 of the 114 quinolone-resistant strains had gyr mutations. All mutant strains had high minimal inhibitory concentration values for the antibiotics. Our results showed the distribution of the strains with mutations in drug resistance-related genes was low in the M. abscessus group. Furthermore, we performed drug susceptibility testing and sequence analyses to determine the characteristics of these genes in the M. abscessus group.
    Annals of Laboratory Medicine 01/2014; 34(1):31-7. DOI:10.3343/alm.2014.34.1.31 · 1.48 Impact Factor
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    • "In particular, a recent study based on whole genome sequencing revealed the first evidence of human to human transmission in NTM infection, by proving its transmission between cystic fibrosis patients; suggesting unusually high infectivity of humans by M. massiliense strains [36]. In South Korea, a distinct epidemiologic trend was reported [37], of higher prevalence of M. massiliense strains (of the M. abscessus − complex); part of which may be attributed to the presence of the M. massiliense Type II genotype found only in Korean patients [20]. "
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    ABSTRACT: Recently, we introduced the complete genome sequence of Mycobacterium massiliense clinical isolates, Asan 50594 belonging to Type II genotype with rough colony morphology. Here, to address the issue of whether the rough colony morphotype of M. massiliense Type II genotype is genetically determined or not, we compared polymorphisms of the glycopeptidolipid (GPL) gene locus between M. massiliense Type II Asan 50594 and other rapidly growing mycobacteria (RGM) strains via analysis of genome databases. We found deletions of 10 genes (24.8 kb), in the GPL biosynthesis related gene cluster of Asan 50594 genome, but no deletions in those of other smooth RGMs. To check the presence of deletions of GPL biosynthesis related genes in Mycobacterium abscessus - complex strains, PCRs targeting 12 different GPL genes (10 genes deleted in Asan 50594 genome as well as 2 conserved genes) were applied into 76 clinical strains of the M. abscessus complex strains [54 strains (Type I: 33, and Type II: 21) of M. massiliense and 22 strains (rough morphoype: 11 and smooth morphotype: 11) of M. abscessus]. No strains of the Type II genotype produced PCR amplicons in a total of 10 deleted GPL genes, suggesting loss of GPL biosynthesis genes in the genome of M. massiliense type II genotype strains. Our data suggested that the rough colony morphotype of the M. massiliense Type II genotype may be acquired via deletion events at the GPL gene locus for evolutionary adaptation between the host and pathogen.
    BMC Genomics 12/2013; 14(1):890. DOI:10.1186/1471-2164-14-890 · 3.99 Impact Factor
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