Article

Early determination of sex in jojoba plant by CAPS assay

The Journal of Agricultural Science (Impact Factor: 2.89). 05/2011; 149(03):327 - 336. DOI: 10.1017/S0021859610000948

ABSTRACT Jojoba [Simmondsia chinensis (Link) Schneider] is a dioecious plant grown for its seeds, which are the source of liquid wax or jojoba oil. The sex of jojoba plants cannot be determined with morphological characters until the plants reach reproductive maturity at 3 or more years old. This difficulty of early sex determination imposes severe constraints in breeding studies and in the sex allocation of seedlings in seed orchard establishment, and importantly in a priori mating designs to produce superior jojoba individuals. This study reports three new cleavage-amplified polymorphic sequence (CAPS) assays, which identify male and female individuals distinctly. One of the assays could also identify hermaphrodite jojoba plants existing in nature or obtained using mutagenesis studies.

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Available from: Mehmet Karaca, Dec 13, 2014
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    • "Amplicons were separated using 2% (Td-DAMD-PCR) and 3% (Td-SSR) high resolution agarose gel (Serva, Heidelberg, Germany) electrophoresis according the procedures described in Ince et al. (2011). Amplified products in gels were visualized on an ultraviolet trans-illuminator to confirm amplifications, molecular mass and any observed length polymorphisms. "
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