Bacteriophages for Detection and Control of Bacterial Pathogens in Food and Food-Processing Environment

Canadian Research Institute for Food Safety, University of Guelph, Guelph, Ontario, Canada. Electronic address: .
Advances in food and nutrition research 10/2012; 67:241-88. DOI: 10.1016/B978-0-12-394598-3.00006-X
Source: PubMed


This chapter presents recent advances in bacteriophage research and their application in the area of food safety. Section 1 describes general facts on phage biology that are relevant to their application for control and detection of bacterial pathogens in food and environmental samples. Section 2 summarizes the recently acquired data on application of bacteriophages to control growth of bacterial pathogens and spoilage organisms in food and food-processing environment. Section 3 deals with application of bacteriophages for detection and identification of bacterial pathogens. Advantages of bacteriophage-based methods are presented and their shortcomings are discussed. The chapter is intended for food scientist and food product developers, and people in food inspection and health agencies with the ultimate goal to attract their attention to the new developing technology that has a tremendous potential in providing means for producing wholesome and safe food.

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Available from: Hany Anany, Jan 13, 2014
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    • "Thus simple and convenient tools that will help in rapid screening to provide quick assessment of sample integrity can prevent the occurrence of disease or outbreak due to these pathogens and is of significant interest (Swaminathan and Feng, 1994) to both government and private agencies. Reports on pathogen detection have usually focused on the following methodologies such as traditional culture collection (Reissbrodt, 2004; Velusamy et al., 2010), genetic approaches based on polymerase chain reaction (PCR) (Bennett et al., 1998; Gallegos-Robles et al., 2009; Malorny et al., 2003), and immunological methods (Cho et al., 2014; Pandey et al., 2014), although other methods such as scattering spectroscopy (Banada et al., 2009; Bhunia, 2008; Rajwa et al., 2010) and phage-based detection (Brovko et al., 2012; Goodridge et al., 1999; Smartt et al., 2012) have also been used. Methods such as colony counting and biochemical methods are effective and reliable and are the gold standard, but takes several days and are accompanied with laborious steps. "
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