Article

Qualitative and quantitative aspects of the serological diagnosis of early syphilis.

Scottish Bacterial Sexually Transmitted Infections Reference Laboratory, Laboratory Medicine (Microbiology), 51 Little France Crescent, Edinburgh, Scotland, UK.
International Journal of STD & AIDS (Impact Factor: 1). 10/2008; 19(9):620-4. DOI: 10.1258/ijsa.2008.008103
Source: PubMed

ABSTRACT The aim of the present study was to evaluate the use of various serological tests in the diagnosis of early syphilis. The Murex enzyme immunoassay (EIA) test was used for screening; the Venereal Diseases Research Laboratory (VDRL) test, the Treponema pallidum particle agglutination assay (TPPA) and the Mercia antitreponemal IgM EIA were used in all the patients with a positive screening test and in those with suspected syphilis or in known contacts. In 89 cases of primary syphilis, the Murex EIA screening test was positive in 67 (75%) patients, the Mercia IgM EIA in 80 (90%) cases, the VDRL in 60 (67%) cases and the TPPA in 85 (96%) cases. All the tests were positive in 68 patients with secondary syphilis. In 72 cases of early latent syphilis, the Murex EIA screening test was positive in 68 (94%) patients, the Mercia IgM EIA in 50 (69%) cases, the VDRL in 61 (85%) cases and the TPPA in 68 (94%) cases. The Mercia IgM EIA was the only test positive in four (6%) of these cases; these four patients were known contacts. Antibody titres in the VDRL and TPPA increased as the infection progressed.

0 Bookmarks
 · 
296 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A global resurgence of yaws in developing countries highlights the need for reliable diagnostic criteria for this neglected infection. We conducted a clinical and serologic survey of 233 children less than 15 years of age who had clinically suspected yaws. A total of 138 (59%) cases were confirmed serologically, and 10 of 12 primary stage cases showed positive results for Treponema pallidum by a polymerase chain reaction assay that has not yet been validated for identification of yaws. A high proportion of cases (46%) were in the secondary stage; 92% of them had osteoarticular involvement, and only 24% had a Venereal Disease Research Laboratory titer greater than 1:32.
    The American journal of tropical medicine and hygiene 07/2011; 85(1):113-6. · 2.53 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We compared two automated Rapid Plasma Reagin (RPR) assay kits with a manual RPR assay kit to evaluate the possibility of using the two automated RPR assays as an alternative to the manual RPR assay for a quantitative monitoring. One hundred eighty-five samples were analyzed, including 16 sera from patients with primary, secondary, and latent syphilis. Measured RPR unit (R.U.) values of two automated RPR assay kits, Mediace RPR (Sekisui Chemical Co., Ltd, Japan) and HBi Auto RPR (HBI Co., Ltd, Korea), were compared with the RPR titers of Macro-Vue RPR card test (Becton Dickinson BD Microbiology systems, USA). As a confirmatory test, Anti-Treponema pallidum EUROLINE WB (IgG) and Anti-Treponema pallidum EUROLINE WB (IgM) (Euroimmun, Germany) were used. There was a prozone effect with Mediace RPR at RPR titer (card test) of 1:16, but not with HBi Auto RPR. The R.U. values of the two automated RPR assays did not show proportional increase to the RPR titer. Agreement between manual RPR and two automated RPR assay kits, Mediace RPR assay and HBi Auto RPR assay, were 83.8% and 83.2%, respectively. The two automated RPR assay kits could not be used as an alternative to manual RPR test for quantitative analysis of RPR titer. As Mediace RPR shows a prozone effect at relatively low RPR titer, caution is needed in the interpretation of the measured values.
    The Korean Journal of Laboratory Medicine 09/2009; 29(4):331-7. · 0.72 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective To identify the demographic characteristics, risk factors and motivations for donating among blood donors with reactive serologic tests for syphilis.Background Post-donation interviews with syphilis seropositive blood donors improve recruitment and screening strategies.Methods This case–control study compares 75 Venereal Disease Research Laboratory (VDRL) > 8, EIA+ (enzyme immunoassay) and FTA-ABS+ (fluorescent treponemal antibody); 80 VDRL−, EIA+ and FTA-ABS+; and 34 VDRL− and EIA− donors between 2004 and 2009. Donors were assessed by their demographic characteristics, sexual behaviour, history of alcohol and illicit drugs use, and motivations to donate.ResultsDonors with VDRL > 8 were more likely to be divorced [AOR = 12·53; 95% confidence interval (CI) 1·30–120·81], to have had more than six sexual partners (AOR=7·1; 95% CI 1·12–44·62) and to report male–male-sex in the past 12 months (AOR=8·18; 95% CI 1·78–37·60). Donors with VDRL−, EIA+ and FTA-ABS+ were less likely to be female (AOR=0·26; 95% CI 0·07–0·96), more likely to be older (AOR=10·2; 95% CI 2·45–42·58 ≥ 39 and <60 years old) and to have had more than six sexual partners in the past 12 months (AOR = 8·37; 95% CI 1·49–46·91). There was no significant difference among groups regarding illicit drugs use; 30·7% (VDRL > 8) and 12·5% (VDRL−, EIA+ and FTA-ABS+) of donors reported that they had been at risk for HIV infection (P = 0·004). One-third of donors came to the blood bank to help a friend or a relative who needed blood.Conclusion Although donors exposed to syphilis reported and recognised some high risk behaviour, most were motivated by direct appeal to donate blood. Monitoring the risk profile of blood donors can benefit public health and improve blood safety.
    Transfusion Medicine 04/2014; · 1.26 Impact Factor

Full-text

View
9 Downloads
Available from