Hypolipidaemic and anti-atherosclerotic effects of lupin proteins in a rabbit model

The British journal of nutrition (Impact Factor: 3.45). 09/2008; 100(04):707 - 710. DOI: 10.1017/S000711450894215X

ABSTRACT The biological activities of a protein isolate from lupin (Lupinus albus) were studied in a rabbit model of atherosclerosis. Focal plaque development was induced at both common carotid arteries by perivascular injury. After surgery, animals were fed three different diets for 90 d, all with 1 % cholesterol, 15 % SFA and 20 % protein; the protein source was casein (CAS), lupin proteins (LUP) or 50 % CAS+50 % LUP (CAS+LUP). Lower cholesterolaemia was detected in the LUP v. the CAS group at 60 and 90 d of treatment ( − 40·3 and − 33·5 %, respectively; P < 0·05). Cryosection analyses of the carotids indicated a significant reduction in focal lesion progression in the LUP v. the CAS group ( − 37·4 %; P < 0·05). In summary, in a rabbit model of atherosclerosis, a protein isolate from L. albus reduced cholesterolaemia and exerted a remarkable protective activity against atherosclerosis progression.

  • [Show abstract] [Hide abstract]
    ABSTRACT: Previous experiments in suitable animal models and in mild hypercholesterolemic individuals have shown that the consumption of lupin proteins may be useful for controlling total and low-density-lipoprotein (LDL) cholesterol levels. With the objective of providing evidences that peptides deriving from the hydrolysis of lupin proteins may be responsible of the observed activities and for investigating the mechanism of action, HepG2 cells were treated with lupin peptides obtained either by pepsin (P) or trypsin (T) hydrolysis and molecular and functional investigations were performed on the LDL receptor / SREBP2 pathway. For the first time, this report provides the experimental evidence that lupin peptides are able to interfere with the HMGCoAR activity, up-regulating the LDL receptor (136% and 84% vs. the control for P and T peptides, respectively, at 1 mg/ml) and SREBP2 proteins (148% and 73% vs. the control for P and T peptides, respectively, at 1 mg/ml) via the activation of PI3K/Akt/GSK3β pathways and increasing the LDL-uptake at HepG2 cell line (40% and 50% vs. the control for P and T peptides, respectively, at 1 mg/ml). These results may be useful to explain the activities observed in vivo in animals and humans treated with lupin protein.
    Journal of agricultural and food chemistry. 06/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Lupin kernel fiber beneficially modifies blood lipids because of its bile acid-binding capacity. The aim of this study was to evaluate the preventive effects of a lupin kernel fiber preparation on cardiovascular diseases and to clarify possible mechanisms. In a randomized, double-blind, controlled crossover trial, 60 moderately hypercholesterolemic adults (plasma total cholesterol: >5.2 mmol/L) passed 3 intervention periods in different orders with a 2-wk washout phase between each. Participants consumed either a high-fiber diet containing 25-g/d lupin kernel fiber (LF) or citrus fiber (CF), or a low-fiber control diet (CD) for 4 wk each. Anthropometric, plasma, and fecal variables were assessed at baseline and after the interventions. Contrary to the CF period, total (9%) and LDL (12%) cholesterol as well as triacylglycerols (10%) were lower after the LF period when compared with the CD period [P ≤ 0.02, adjusted for baseline, age, gender, and body mass index (BMI)]. HDL cholesterol remained unchanged. Moreover, the LF period reduced high-sensitivity C-reactive protein (P = 0.02) and systolic blood pressure (P = 0.01) when compared with baseline. Bile acid binding could not be shown because the excretion of total bile acids remained constant after the high-fiber diets. However, the LF period resulted in an enhanced formation of the main short-chain fatty acids in comparison with the CD period. During the CF period, only acetate increased significantly. Both high-fiber diets led to higher satiety and modified nutritional behavior, resulting in significantly lower body weight, BMI, and waist circumference compared with the CD period. The blood lipid-lowering effects of LF are apparently not a result of bile acid binding. Rather, we hypothesize for the first time that the blood lipid-lowering effects of LF may be mainly attributed to the formation of short-chain fatty acids, specifically propionate and acetate. This trial was registered at as NCT01035086.
    Journal of Nutrition 02/2014; · 4.23 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The seeds of the plants of the Fabaceae, commonly known as “grain legumes” or “pulses,” are major foodstuffs in most countries. In addition, these seeds may also provide some health benefits, in particular in the area of hypercholesterolemia and hypertension prevention. Whereas the hypocholesterolemic activity of soy protein has been well known for decades and was finally supported by the health claim by the U.S. Food and Drug Administration in 1999, similar information on non-soy legumes is scarce. This paper reviews all such available data from animal models and human trials as well as information on the mechanism of action provided by in vitro studies, mainly on cell cultures or assays on specific enzymes. This body of data indicates that a regular consumption of grain legumes may be useful both for the prevention of hypercholesterolemia and hypertension. More investigations are needed, however, for elucidating the mechanism of action and the actual effective components in legumes.
    Critical Reviews in Plant Sciences 06/2015; 34. · 5.29 Impact Factor

Full-text (2 Sources)

Available from
Jun 1, 2014