Comparison of the diversity of the vaginal microbiota in HIV-infected and HIV-uninfected women with or without bacterial vaginosis.

Department of Immunology/Microbiology, Rush University Medical Center, Chicago, Illinois, USA.
The Journal of Infectious Diseases (Impact Factor: 5.78). 09/2008; 198(8):1131-40. DOI: 10.1086/591942
Source: PubMed

ABSTRACT Whether human immunodeficiency virus (HIV) infection is associated with a change in the diversity of genital microbiota in women was investigated.
Amplicon length heterogeneity polymerase chain reaction (LH-PCR) analysis and pyrosequencing of the 16S ribosomal RNA gene were used to analyze the diversity of the microbiota in HIV-positive (HIV(+)) and HIV-negative (HIV(-)) women with or without bacterial vaginosis (BV).
LH-PCR analysis revealed significantly more microbiota diversity in BV-positive (BV(+)) women than in BV-negative (BV(-)) women, but no significant difference was noted between HIV(+) women and HIV(-) women. Pyrosequencing revealed that Lactobacillus organisms constituted a median of 96% of the bacteria in BV(-) women. BV(+) women had a significantly higher number of taxa found at > or =1% of the total genital microbiota (median, 11 taxa). Common taxa in BV(+) women were Prevotella, Megasphaera, Gardnerella, Coriobacterineae, Lachnospira, and Sneathia. There was a trend (P = .07) toward the presence of a higher number of taxa in HIV(+)BV(+) women than in HIV(-)BV(+) women. Propionibacterineae, Citrobacter, and Anaerococcus were the taxa found only in HIV(+) women (P < .05).
The present study demonstrated that both LH-PCR analysis and pyrosequencing differentiated microbiota in BV(+) women from that in BV(-) women and that pyrosequencing indicated a trend toward increased diversity in BV(+)HIV(+) women, suggesting that HIV infection is associated with changes in the diversity of genital microbiota.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: To identify the microbiota communities in the vaginal tracts of healthy Mexican women across the pregnancy. Vaginal swabs were obtained during the prenatal visit of women from all trimesters (n = 64) of healthy pregnant women of Mexico City. DNA was isolated from each sample, and PCR-DGGE and sequencing of 16S rRNA gene fragments were used to identify the bacterial communities. 21 different microorganisms were identified in the vaginal samples. Lactobacillus genus was present in 98% of women studied. Four lactobacilli species were identified in vaginal samples. L. acidophilus was the predominant (78%) followed by L. iners (54%), L. gasseri (20%), and L. delbrueckii (6%). 17 different microorganisms related to bacterial vaginosis conditions were identified. Ureaplasma urealyticum was the predominant (21%) followed by BVAB1 (17%) and Gemella bergeriae (7.8%). Lactobacillus genus predominates in the vaginal samples of Mexican pregnant women associated with different microorganisms related to bacterial vaginosis conditions.
    Infectious Diseases in Obstetrics and Gynecology 09/2011; 2011:851485. DOI:10.1155/2011/851485
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: As a result of advancements in high-throughput technology, the sequencing of the pioneering 16S rRNA gene marker is gradually shedding light on the taxonomic characterization of the spectacular microbial diversity that inhabits the earth.16S rRNA-based investigations of microbial environmental niches are currently conducted using several technologies, including large-scale clonal Sanger sequencing, oligonucleotide microarrays, and, particularly, 454 pyrosequencing that targets specific regions or is linked to barcoding strategies. Interestingly, the short readlength produced by next-generation sequencing technology has led to new computational efforts in the taxonomic sequence assignment process.From a medical perspective, the characterization of the microbial composition of the skin surface, oral cavity, and gut in both healthy and diseased people enables a comparison of microbial community profiles and also contributes to the understanding of the potential impact of a particular microbial community.
    Journal of Computer Science & Systems Biology 02/2009; DOI:10.4172/jcsb.1000019
  • [Show abstract] [Hide abstract]
    ABSTRACT: Humans live in association with abundant, complex, and dynamic microbial populations (the microbiome) that colonize many body sites, including the vaginal tract. Interactions between the host and the vaginal microbiota greatly affect women’s health, where they often serve a protective role in maintaining vaginal health. Disruption of the microbial composition can lead to increased susceptibility to various urogenital diseases, including bacterial vaginosis (BV), vulvovaginal candidiasis (VVC), pelvic inflammatory disease (PID), and sexually transmitted diseases (STDs) such as infection with Chlamydia, Trichomonas, and human immunodeficiency virus (HIV). The composition of the vaginal microbiota also has a notable impact on pregnancy and neonatal outcome, including complications such as preterm labor and delivery. Understanding the composition and dynamics of the vaginal ecosystem as well as the involvement of metabolic and immunologic components is an area of increasing interest and research.
    01/1970: pages 91-115;


Available from