Article

BM-derived cells randomly contribute to neoplastic and non-neoplastic epithelial tissues at low rates

Institute of Pathology, Locarno, Switzerland.
Bone marrow transplantation (Impact Factor: 3). 09/2008; 42(11):749-55. DOI: 10.1038/bmt.2008.243
Source: PubMed

ABSTRACT Epithelial cancers can arise from BM-derived cells (BMCs) in animal models. We studied whether the same phenomenon can occur in humans. Biopsy specimens from carcinomas and healthy adjacent tissues were obtained from three women who had undergone allogeneic BMT from an HLA-matched brother. Complete donor hematopoietic chimerism was verified by cytogenetic analysis, RFLP analysis or by reverse transcription-PCR analysis. Biopsies were studied for the presence of the Y chromosome derived from BM-derived cells by combined FISH and immunohistochemical staining. In our studies, we showed that human epithelial neoplastic and adjacent non-neoplastic tissues incorporate the Y chromosome at low and comparable rates. The lack of enrichment in malignancies argues against the possibility that BM-derived cells represent a direct source of carcinomas, and we suggest that these cells randomly contribute to neoplastic and non-neoplastic epithelial cells. On the basis of the absence of a fusion karyotype, we favor a model in which the differentiation of BM-derived cells is largely determined by the microenvironment encountered.

0 Bookmarks
 · 
122 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Secondary solid tumors that occur after hematopoietic stem cell transplantation (HSCT) are late complications of HSCT. Previously, secondary solid tumors were considered to be recipient-derived cells because transplanted cells do not contain epithelial cells. Recently, however, not only donor‑derived epithelial cells but also donor-derived secondary solid tumors have also been reported in mice and humans. It means that circulating bone marrow-derived stem cells (BMDCs) including hematopoietic stem cells include the stem cells of many tissue types and the precancerous cells of many solid tumors. In most reports of donor-derived secondary solid tumors, however, tumors contained a low proportion of BMDC-derived epithelial cells in mixed solid tumor tissues. To our knowledge, there are only five known cases of completely donor-derived tumor tissues, i.e., four oral SCCs and a pharyngeal SCC. In this study, we analyzed five human clinical samples of solid tumors, i.e., two esophageal squamous cell carcinomas (SCCs), two oral SCCs and a tongue carcinoma. In the oral and tongue, completely donor-derived tissues were not observed, but in esophagus a completely donor-derived esophageal epidermis and SCC were observed for the first time. In addition, in another esophageal SCC patient, a completely donor-derived dysplasia region of esophageal epidermis was observed near recipient-derived SCC. This study suggests that BMDC-derived cells include the stem cells of esophageal epidermis and the precancerous cells of esophageal SCC and can differentiate into esophageal epithelium and esophageal SCC.
    International Journal of Oncology 12/2013; 44(2). DOI:10.3892/ijo.2013.2206 · 2.77 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Beam-based alignment is essential for the operation of a SASE-FEL at the TESLA Test Facility Linac. It requires the transverse beam position to be measured at several points inside and between the undulator modules with a resolution of better than 5 μm. Between the undulator modules cylindrical cavities will be used, excited in the TM <sub>110</sub>-mode by an off-center beam. The amplitude of this mode is detected in a homodyne receiver by mixing the cavity output and a 12 GHz reference signal. For monitors inside the undulator modules the realization of two different concepts is under way. The first one is a button-type monitor using very small feedthroughs. The second one is a structure consisting of four ridged waveguides oppositely arranged around the chamber, where the averaged position of a single bunch train will be measured in a narrowband X-band receiver. The fabrication and test of prototypes is under way. This paper summarizes the designs and some preliminary results
    Particle Accelerator Conference, 1997. Proceedings of the 1997; 06/1997
  • [Show abstract] [Hide abstract]
    ABSTRACT: The currently prevalent somatic mutation theory of carcinogenesis and metastases explicitly assumes that cancer is a cellular disease, i.e. a disease of the control of cell proliferation and/or cell differentiation. Accordingly, explanations should always be sought for at a gene and/or gene product level, regardless of the level of organization at which the phenomenon is observed. Such a reductionist approach characterized the century-old effort to find cancer cell singularities, absent in normal cells, without apparent success, however. More recently alternative views have been put forward, assuming that cancer is a tissue-based disease involving disturbed interactions within the tissue architecture. In this review, selected reports on normal tissue homeostasis and bone marrow contribution to both tumour cells and tumour stroma are reviewed. Regarding normal tissues, the existence of a complex homeostatic system actually involving the whole organism emerges. Regarding tumours, remarkable similarities with normal tissue activities are apparent, providing some evidence that tumours share many biological features and processes with normal tissues. The review supports the concept that cancer is a tissue-based disease and that its pathological nature may result from unbalanced/untimely activation of otherwise normal physiological processes.
    Targeted Oncology 05/2013; 8(2). DOI:10.1007/s11523-013-0277-6 · 3.46 Impact Factor

Full-text

Download
79 Downloads
Available from
Jul 24, 2014