Phytochemical composition and in vitro pharmacological activity of two rose hip (Rosa canina L.) preparations

Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria.
Phytomedicine: international journal of phytotherapy and phytopharmacology (Impact Factor: 2.88). 09/2008; 15(10):826-35. DOI: 10.1016/j.phymed.2008.06.012
Source: PubMed

ABSTRACT The aim of the present study was to compare powdered rose hip with and without fruits (Rosae pseudofructus cum/sine fructibus, Rosa canina L., Rosaceae) with regard to their phytochemical profile and their in vitro anti-inflammatory and radical-scavenging properties. The two powders were subsequently extracted with solvents of increasing polarity and tested for inhibition of cyclooxygenase (COX-1, COX-2) and of 5-LOX-mediated leukotriene B(4) (LTB(4)) formation as well as for DPPH-radical-scavenging capacity. While the water and methanol extracts were inactive in the COX-1, COX-2 and LTB(4) inhibition assays, the n-hexane and the dichloromethane extracts inhibited all three enzymes. In the active extracts, the triterpenoic acids ursolic acid, oleanolic acid and betulinic acid were identified, although only in minute amounts. Furthermore, oleic, linoleic and alpha-linolenic acid were identified apart from several saturated fatty acids. Even though unsaturated fatty acids are known to be good inhibitors of COX-1, COX-2 and LT formation, no clear correlation between their concentration in the extracts and their activity was found. We suggest that other, yet unidentified, lipophilic constituents might play a more important role for the observed in vitro inhibitory activity on arachidonic acid metabolism. Some of the extracts also showed considerable DPPH radical scavenging activity, the methanolic extracts being most potent. The radical scavenging activity of the extracts correlated very well with their total phenolic content, while ascorbic acid contributes only little to the radical-scavenging activity due to its low concentration present in the extracts. In summary, extracts derived from powdered rose hip without fruits were more effective in all assays carried out compared with extracts derived from powdered rose hip with fruits.

1 Follower
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The crude methanol extracts and fractions of the root and stem barks of Dillenia serrata Thunb. showed 64% to 73% inhibition on the production of prostaglandin E2 (PGE2) in lipopolysaccharide-induced human whole blood using a radioimmunoassay technique. Three triterpenoids isolated from the root bark of the plant, koetjapic (1), 3-oxoolean-12-en-30-oic (2), and betulinic (3) acids, exhibited significant concentration-dependent inhibitory effects on PGE2 production with IC50 values of 1.05, 1.54, and 2.59 μM, respectively, as compared with the positive control, indomethacin (IC50 = 0.45 μM). Quantification of compounds 1 and 3 in the methanol extracts and fractions were carried out by using a validated reversed-phase high performance liquid chromatography (RP-HPLC) method. The ethyl acetate fraction of the stem bark showed the highest content of both compound 1 (15.1%) and compound 3 (52.8%). The strong inhibition of the extracts and fractions on cyclooxygenase-2 (COX-2) enzymatic activity was due to the presence of their major constituents, especially koetjapic and betulinic acids.
    Molecules 02/2015; 20(2):3206-3220. DOI:10.3390/molecules20023206 · 2.10 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: RESUMEN Las condiciones históricas, climatológicas y edáficas del País Vasco, son las principales causas de que su extensión se vea dominada por 3 formaciones vegetales potenciales: robledales, hayedos y quejigales. Nuestro objetivo fue estudiar qué especies vegetales medicinales presentan evidencias científicas para tratar problemas de sueño. Para ello, se con-sultó bibliografía y bases de datos referentes a especies sedantes, hipnóticas o ansiolíticas por presentar mecanismos de actuación similares, así como sus principios activos. Se concluye el estudio con la catalogación de 22 especies vegetales del País Vasco como remedios naturales para combatir los problemas del sueño presentes en la sociedad. ABSTRACT Historic, climatic and soil conditions of the Basque Country, are the main causes which provokes its extension is mainly dominated by 3 types of potential vegetation: oak, beech and gall. Our objective was focused in researching what plants show scientific evidences to be used within sleep problems. Scientific literature and databases were consulted in those species referenced as sedatives, hypnotics or anxiolytics due they act through similar mechanisms, their bioactive prin-ciples were revised too. We conclude this study with 22 plant species in Basque Country as natural sleep inducers to be used in sleep problems in our society. Correspondencia: Lierni Ugartemendia Ugalde: (Titulada en Nutrición Humana y Dietética) Rafael Bravo Santos: (Licenciado en Biología con Grado y Máster)
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Asthma is one of the most common chronic disorders and about 7% of the US population suffers from asthma. Initial response to allergy is associated with mediators like histamine, prostaglandin (PG) D2 and leukotriene (LT) C4. In asthmatic patients, 5-lipo-oxygenase (5-LO) pathway produces more LTC4 than the healthy people. Also, pro-inflammatory factors like interleukin (IL)-1, IL-6 and Tumor Necrosis Factor (TNF)-α play a remarkable role in bronchoconstriction. The common asthma medications are associated with a number of unwanted side effects, especially throughout long term treatment. Herbal medication is an alternative treatment for asthma and the plants are rich sources of antioxidants that intensively reduce the activity of free radicals. Among several herbs, Rosa canina (RC) is a potent anti-inflammatory remedy which contains a number of well-known antioxidants including vitamin C, quercetin, hesperidin, rutin and some other therapeutic compounds. The aim of this study was to review the scientific evidences supporting the probable influence of Rosa canina on asthma.
    Advances in Bioscience and Clinical Medicine 09/2014; 3(1):48-52. DOI:10.7575/aiac.abcmed.

Full-text (2 Sources)

Available from
May 26, 2014