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Phytochemical composition and in vitro pharmacological activity of two rose hip (Rosa canina L.) preparations

Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria.
Phytomedicine: international journal of phytotherapy and phytopharmacology (Impact Factor: 2.88). 09/2008; 15(10):826-35. DOI: 10.1016/j.phymed.2008.06.012
Source: PubMed

ABSTRACT The aim of the present study was to compare powdered rose hip with and without fruits (Rosae pseudofructus cum/sine fructibus, Rosa canina L., Rosaceae) with regard to their phytochemical profile and their in vitro anti-inflammatory and radical-scavenging properties. The two powders were subsequently extracted with solvents of increasing polarity and tested for inhibition of cyclooxygenase (COX-1, COX-2) and of 5-LOX-mediated leukotriene B(4) (LTB(4)) formation as well as for DPPH-radical-scavenging capacity. While the water and methanol extracts were inactive in the COX-1, COX-2 and LTB(4) inhibition assays, the n-hexane and the dichloromethane extracts inhibited all three enzymes. In the active extracts, the triterpenoic acids ursolic acid, oleanolic acid and betulinic acid were identified, although only in minute amounts. Furthermore, oleic, linoleic and alpha-linolenic acid were identified apart from several saturated fatty acids. Even though unsaturated fatty acids are known to be good inhibitors of COX-1, COX-2 and LT formation, no clear correlation between their concentration in the extracts and their activity was found. We suggest that other, yet unidentified, lipophilic constituents might play a more important role for the observed in vitro inhibitory activity on arachidonic acid metabolism. Some of the extracts also showed considerable DPPH radical scavenging activity, the methanolic extracts being most potent. The radical scavenging activity of the extracts correlated very well with their total phenolic content, while ascorbic acid contributes only little to the radical-scavenging activity due to its low concentration present in the extracts. In summary, extracts derived from powdered rose hip without fruits were more effective in all assays carried out compared with extracts derived from powdered rose hip with fruits.

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    • "To the best of our knowledge, many studies have been conducted to investigate rheological properties of some fruit based products (jams and puree) (Igual et al. 2014; Basu et al. 2013; Falguera et al. 2010). The studies on rose hip fruits have solely focused on chemical composition and their functional properties (Demir et al. 2014; Andersson et al. 2011; Wenzig et al. 2008). However, no study has been reported so far in the literature on phenolic content and steady/dynamic rheological properties of the rose hip marmalade. "
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    ABSTRACT: In this study, bioactive (total phenolic, antioxidant and antiradical activity) and rheological properties (steady and dynamic) of rose hip marmalade were investigated. Bioactive properties were determined in rose hip marmalade and extract. Extract had higher total phenolic content (38.5 mg GAE/g dry extract), antioxidant activity (124 mg AAE/g dry extract) and antiradical activity (49.98 %) than marmalade. Steady and dynamic rheological properties of the marmalade were determined at different temperature levels (5, 25 and 45 °C). Rose hip marmalade exhibited shear thinning behavior and Ostwald de Waele model best described flow behavior of the sample (R 2 ≥ 0.9880) at different temperature levels. Consistency index and apparent viscosity values (η 50 ) at shear rate 50 s−1 decreased with increase in temperature level. Viscoelastic properties were determined by oscillatory shear measurements and G' (storage modulus) values were found to be higher than G'' (loss modulus) values, indicating that the rose hip marmalade had a weak gel−like structure with solid-like behavior. G', G'', G * (complex modulus) and η* (complex viscosity) values decreased with increase in temperature level. Modified Cox-Merz rule was satisfactorily applied to correlate apparent and complex viscosity values of the rose hip marmalade at all temperatures studied.
    Journal of Food Science and Technology -Mysore- 01/2015; DOI:10.1007/s13197-015-1753-z · 2.02 Impact Factor
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    • "Oznaczenie wykonywano wg Wenzig i wsp. [24]. W probówce umieszczano 2,5 ml etanolowego ekstraktu analizowanej próbki oraz 7,5 ml roztworu DPPH, następnie po 30 min od zainicjowania reakcji mierzono absorbancję. "
    Zywnosc: Nauka, Technologia, Jakosc 12/2012; 19(4):32-43. · 0.31 Impact Factor
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    • "The major bioactive compounds within rose hips are phenols, ascorbic acid, tocopherols, β-carotene, lycopene, tannins, pectins, sugars, organic acids, amino acids and essential fatty acids [30] [31]. Other rose-hip-derived compounds reported include galactolipids [31] and triterpenic acids [32]. Interestingly, the antioxidant effects of these compounds cannot fully account for the clinical effects of rose hip powder [33] and the efficacy of the phenolic compounds in the rose hips has yet to be evaluated in controlled clinical trials [30]. "
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    ABSTRACT: Rose hips are popular in health promoting products as the fruits contain high content of bioactive compounds. The aim of this study was to investigate whether health benefits are attributable to ascorbic acid, phenols, or other rose-hip-derived compounds. Freeze-dried powder of rose hips was preextracted with metaphosphoric acid and the sample was then sequentially eluted on a C(18) column. The degree of amelioration of oxidative damage was determined in an erythrocyte in vitro bioassay by comparing the effects of a reducing agent on erythrocytes alone or on erythrocytes pretreated with berry extracts. The maximum protection against oxidative stress, 59.4 ± 4.0% (mean ± standard deviation), was achieved when incubating the cells with the first eluted meta-phosphoric extract. Removal of ascorbic acid from this extract increased the protection against oxidative stress to 67.9 ± 1.9%. The protection from the 20% and 100% methanol extracts was 20.8 ± 8.2% and 5.0 ± 3.2%, respectively. Antioxidant uptake was confirmed by measurement of catechin by HPLC-ESI-MS in the 20% methanol extract. The fact that all sequentially eluted extracts studied contributed to protective effects on the erythrocytes indicates that rose hips contain a promising level of clinically relevant antioxidant protection.
    Oxidative Medicine and Cellular Longevity 07/2012; 2012:621579. DOI:10.1155/2012/621579 · 3.36 Impact Factor
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