Phytochemical composition and in vitro pharmacological activity of two rose hip (Rosa canina L.) preparations

Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria.
Phytomedicine: international journal of phytotherapy and phytopharmacology (Impact Factor: 3.13). 09/2008; 15(10):826-35. DOI: 10.1016/j.phymed.2008.06.012
Source: PubMed


The aim of the present study was to compare powdered rose hip with and without fruits (Rosae pseudofructus cum/sine fructibus, Rosa canina L., Rosaceae) with regard to their phytochemical profile and their in vitro anti-inflammatory and radical-scavenging properties. The two powders were subsequently extracted with solvents of increasing polarity and tested for inhibition of cyclooxygenase (COX-1, COX-2) and of 5-LOX-mediated leukotriene B(4) (LTB(4)) formation as well as for DPPH-radical-scavenging capacity. While the water and methanol extracts were inactive in the COX-1, COX-2 and LTB(4) inhibition assays, the n-hexane and the dichloromethane extracts inhibited all three enzymes. In the active extracts, the triterpenoic acids ursolic acid, oleanolic acid and betulinic acid were identified, although only in minute amounts. Furthermore, oleic, linoleic and alpha-linolenic acid were identified apart from several saturated fatty acids. Even though unsaturated fatty acids are known to be good inhibitors of COX-1, COX-2 and LT formation, no clear correlation between their concentration in the extracts and their activity was found. We suggest that other, yet unidentified, lipophilic constituents might play a more important role for the observed in vitro inhibitory activity on arachidonic acid metabolism. Some of the extracts also showed considerable DPPH radical scavenging activity, the methanolic extracts being most potent. The radical scavenging activity of the extracts correlated very well with their total phenolic content, while ascorbic acid contributes only little to the radical-scavenging activity due to its low concentration present in the extracts. In summary, extracts derived from powdered rose hip without fruits were more effective in all assays carried out compared with extracts derived from powdered rose hip with fruits.

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    • "To the best of our knowledge, many studies have been conducted to investigate rheological properties of some fruit based products (jams and puree) (Igual et al. 2014; Basu et al. 2013; Falguera et al. 2010). The studies on rose hip fruits have solely focused on chemical composition and their functional properties (Demir et al. 2014; Andersson et al. 2011; Wenzig et al. 2008). However, no study has been reported so far in the literature on phenolic content and steady/dynamic rheological properties of the rose hip marmalade. "
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    ABSTRACT: In this study, bioactive (total phenolic, antioxidant and antiradical activity) and rheological properties (steady and dynamic) of rose hip marmalade were investigated. Bioactive properties were determined in rose hip marmalade and extract. Extract had higher total phenolic content (38.5 mg GAE/g dry extract), antioxidant activity (124 mg AAE/g dry extract) and antiradical activity (49.98 %) than marmalade. Steady and dynamic rheological properties of the marmalade were determined at different temperature levels (5, 25 and 45 °C). Rose hip marmalade exhibited shear thinning behavior and Ostwald de Waele model best described flow behavior of the sample (R 2 ≥ 0.9880) at different temperature levels. Consistency index and apparent viscosity values (η 50 ) at shear rate 50 s−1 decreased with increase in temperature level. Viscoelastic properties were determined by oscillatory shear measurements and G' (storage modulus) values were found to be higher than G'' (loss modulus) values, indicating that the rose hip marmalade had a weak gel−like structure with solid–like behavior. G', G'', G * (complex modulus) and η* (complex viscosity) values decreased with increase in temperature level. Modified Cox-Merz rule was satisfactorily applied to correlate apparent and complex viscosity values of the rose hip marmalade at all temperatures studied.
    Journal of Food Science and Technology -Mysore- 03/2015; 52(10). DOI:10.1007/s13197-015-1753-z · 2.20 Impact Factor
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    • "The radical scavenging activity of the methanolic Rosa canina L. extracts was determined from its DPPH radical quenching ability. Some studies showed that antioxidant activity of plants extracts is correlated with total phenolics rather than with individual phenolic compound [25], so the total phenol content was investigated in this study. It is important to state that different phenols develop different activities, depending on their chemical structure (phenolic acids, flavonols, antocyanidins, stilbens) and the capacity for scavenging free radicals from these classes of compounds differs. "
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    ABSTRACT: Background The theoretical, but especially the practical values of identifying the biochemical compounds from the Rosa canina L. fruits are of present interest, this aspect being illustrated by the numerous researches. It was reported that the Rosa canina L. fruit, with its high ascorbic acid, phenolics and flavonoids contents, have antioxidant, antimutagenic and anticarcinogenic effects. This study was performed on order to evaluate the amount of the main phytochemicals (vitamin C, total polyphenols, and total flavonoids) content and their antioxidant activity. Results The results obtained revealed that the average amounts of vitamin C within the studied genotypes were: 360.22 mg/100 g frozen pulp (var. transitoria f. ramosissima, altitude 1250 m) and 112.20 mg/100 g frozen pulp (var. assiensis, altitude 440 m), giving a good correlation between the vitamin C content of the rosehip and the altitude. The total polyphenols content varied from 575 mg/100 g frozen pulp (var. transitoria f. ramosissima) to 326 mg/100 g frozen pulp (var. lutetiana f. fallens). The total flavonoids content showed the highest value for var. assiensis variant 163.3 mg/100 g frozen pulp and the lowest value attributed to var. transitoria f. montivaga 101.3 mg/100 g frozen pulp. The antioxidant activity of eight rose hip extracts from wild Transylvania populations was investigated through DPPH method. The antioxidant activity revealed a good correlation only with vitamin C content and total polyphenols. Conclusion Eight Rose hip fruit species were compared taking into consideration the ascorbic acid, total polyphenols, total flavonoids contents and their antioxidant activity. Based on these results, two of the rosehip genotypes that were analysed could be of perspective for these species’ amelioration, due to their content of phytochemicals mentioned above. These varieties are var. transitoria f. ramosissima (Bistrita-Nasaud, Agiesel) and var. transitoria f. montivaga (Bistrita-Nasaud, Salva) which can be used as a potential source of natural antioxidants.
    Chemistry Central Journal 04/2013; 7(1):73. DOI:10.1186/1752-153X-7-73 · 2.19 Impact Factor
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    • "Oznaczenie wykonywano wg Wenzig i wsp. [24]. W probówce umieszczano 2,5 ml etanolowego ekstraktu analizowanej próbki oraz 7,5 ml roztworu DPPH, następnie po 30 min od zainicjowania reakcji mierzono absorbancję. "

    Zywnosc: Nauka, Technologia, Jakosc 12/2012; 19(4):32-43. · 0.31 Impact Factor
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