Identification of differentially expressed proteins in the cervical mucosa of HIV-1-resistant sex workers

Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2.
Journal of Proteome Research (Impact Factor: 5). 09/2008; 7(10):4446-54. DOI: 10.1021/pr800406r
Source: PubMed

ABSTRACT Novel tools are necessary to understand mechanisms of altered susceptibility to HIV-1 infection in women of the Pumwani Sex Worker cohort, Kenya. In this cohort, more than 140 of the 2000 participants have been characterized to be relatively resistant to HIV-1 infection. Given that sexual transmission of HIV-1 occurs through mucosal surfaces such as that in the cervicovaginal environment, our hypothesis is that innate immune factors in the genital tract may play a role in HIV-1 infection resistance. Understanding this mechanism may help develop microbicides and/or vaccines against HIV-1. A quantitative proteomics technique (2D-DIGE: two-dimensional difference in-gel electrophoresis) was used to examine cervical mucosa of HIV-1 resistant women ( n = 10) for biomarkers of HIV-1 resistance. Over 15 proteins were found to be differentially expressed between HIV-1-resistant women and control groups ( n = 29), some which show a greater than 8-fold change. HIV-1-resistant women overexpressed several antiproteases, including those from the serpin B family, and also cystatin A, a known anti-HIV-1 factor. Immunoblotting for a selection of the identified proteins confirmed the DIGE volume differences. Validation of these results on a larger sample of individuals will provide further evidence these biomarkers are associated with HIV-1 resistance and could help aid in the development of effective microbicides against HIV-1.

  • Salud publica de Mexico 12/2008; 51:s386-s394. DOI:10.1590/S0036-36342009000900004 · 0.94 Impact Factor
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    The Open AIDS Journal 09/2014; 8(1):21-4. DOI:10.2174/1874613601408010021
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    ABSTRACT: Resumen La salud pública en el nuevo milenio tiene como reto integrar los avances de la genómica al derecho fundamental de la salud de todos los seres humanos. La proteómica, entendida como la disciplina científica que estudia los proteomas, es de vital importancia en la investigación en salud, ya que el conocimiento de las proteínas y moléculas efectoras de la función celular permitirá un mejor entendimiento de la fisio-logía humana. En este trabajo se describen los antecedentes y los conocimientos básicos del análisis proteómico basado en la espectrometría de masas y se comentan los usos de la proteómica en la búsqueda de biomarcadores para el diag-nóstico y pronóstico de diferentes enfermedades, los avances en la comprensión de los trastornos crónicos y algunas en-fermedades infecciosas. De manera adicional, se delinean las ventajas de la espectrometría de masas en la genotipificación de patógenos y el estudio de los polimorfismos de una sola base (SNP, por sus siglas en inglés). Palabras clave: proteómica; espectrometría de masas; salud pública Pando-Robles RV, Lanz-Mendoza H. The significance of proteomics in public health. Salud Publica Mex 2009;51 suppl 3:S386-S394. Abstract The proteome is defined as the entirety of proteins expressed by a genome in a given time under specific physiological con-ditions. In an organism, the cells contain the same genome; however, they express different proteins in response to a specific micro-environment. Proteomics is responsible for the study of proteomes, using a wide range of methodologi-cal techniques. Actually, proteomics is a key tool in health research because it has made possible systematic analysis of hundreds of proteins in clinical samples with the promise of discovering new protein biomarkers for different disease conditions. Finally, proteomic strategy is a technology well-suited to provide a better understanding of systems biology and human health.

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