Inkjet-Printed Microfluidic Multianalyte Chemical Sensing Paper

Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
Analytical Chemistry (Impact Factor: 5.64). 09/2008; 80(18):6928-34. DOI: 10.1021/ac800604v
Source: PubMed


This paper presents an inkjet printing method for the fabrication of entire microfluidic multianalyte chemical sensing devices made from paper suitable for quantitative analysis, requiring only a single printing apparatus. An inkjet printing device is used for the fabrication of three-dimensional hydrophilic microfluidic patterns (550-mum-wide flow channels) and sensing areas (1.5 mm x 1.5 mm squares) on filter paper, by inkjet etching, and thereby locally dissolving a hydrophobic poly(styrene) layer obtained by soaking of the filter paper in a 1 wt % solution of poly(styrene) in toluene. In a second step, the same inkjet printing device is used to print "chemical sensing inks", comprising the necessary reagents for colorimetric analytical assays, into well-defined areas of the patterned microfluidic paper devices. The arrangement of the patterns, printed inks, and sensing areas was optimized to obtain homogeneous color responses. The results are "all-inkjet-printed" chemical sensing devices for the simultaneous determination of pH, total protein, and glucose in clinically relevant concentration ranges for urine analysis (0.46-46 muM for human serum albumin, 2.8-28.0 mM for glucose, and pH 5-9). Quantitative data are obtained by digital color analysis in the L*a*b* color space by means of a color scanner and a simple computer program.

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    • "In addition, inkjet-printed electronics can be easily bonded with microÀuidic channels using appropriate inkjet printable materials such as SU-8. Inkjet-printed microÀuidic papers have been introduced for chemical sensing and diagnosing applications [4], [5]. Chemical or biochemical sensors have been realized by using capacitive electrodes. "
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    ABSTRACT: In this letter, a novel fluid-reconfigurable advanced and delayed phase line using a microfluidic composite right/left-handed (CRLH) transmission line (TL) is proposed. A CRLH-TL prototype is inkjet-printed on a photo-paper substrate. In addition, a laser-etched microfluidic channel in poly(methyl methacrylate) (PMMA) is integrated with the CRLH TL using inkjet-printed SU-8 as a bonding material. The proposed TL provides excellent phase-tuning capability that is dependent on the fluidic materials used. As the fluid is changed, the proposed TL can have negative-, zero-, and positive-phase characteristics at 900 MHz for different fluids. The performance of the TL is successfully validated using simulation and measurement results.
    IEEE Microwave and Wireless Components Letters 02/2015; 25(2):142-144. DOI:10.1109/LMWC.2014.2382685 · 1.70 Impact Factor
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    • "The color changes in the chemical sensing areas have been monitored by the naked eye and measured digitally with specific software after recording a color scan. The patterned paper fabricated by photolithography has been reproduced for biological assays by adding appropriate reagents to the testing areas [5] [6]. "
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    ABSTRACT: A novel textile-based analytical device with a simultaneous, rapid, sensitive and qualitative response for analyte detection that may have a potential use in different body fluids such as sweat, blood, saliva and urine is proposed in this study as an alternative to its paper-based counterparts. A porous polypropylene spunbond nonwoven was used as base fabric which is superior to paper with higher tear and crinkle resistance, flexibility and wearability.
    01/2014; 490-491:274-279. DOI:10.4028/
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    • "dependence on sophisticated clinical infrastructure, long turnaround time and relatively high costs. To invent new technologies suitable for use at the point of care and/or in resource limited settings, microfluidic paper-based analytical devices (µPADs) have emerged as powerful diagnostic tools by virtue of their low cost, portability and ease of operation [1] [2] [3] [4] [5]. MicroPADs comprise single or multiple layers of paper substrates with hydrophilic paper channels patterned by hydrophobic materials, and can transport fluids autonomously for performing single-or multi-step analytical assays and quantifying concentrations of various analytes in human fluids (e.g. "
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    ABSTRACT: Paper-based microfluidic devices have emerged as simple yet powerful platforms for performing low-cost analytical tests. This paper reports a microfluidic paper-based electrochemical biosensor array for multiplexed detection of physiologically relevant metabolic biomarkers. Different from existing paper-based electrochemical devices, our device includes an array of eight electrochemical sensors and utilizes a handheld custom-made electrochemical reader (potentiostat) for signal readout. The biosensor array can detect several analytes in a sample solution and produce multiple measurements for each analyte from a single run. Using the device, we demonstrate simultaneous detection of glucose, lactate and uric acid in urine, with analytical performance comparable to that of the existing commercial and paper-based platforms. The paper-based biosensor array and its electrochemical reader will enable the acquisition of high-density, statistically meaningful diagnostic information at the point of care in a rapid and cost-efficient way.
    Science and Technology of Advanced Materials 10/2013; 14(5):4402-. DOI:10.1088/1468-6996/14/5/054402 · 3.51 Impact Factor
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